与墨西哥类病综合体相关的病毒的分布和宿主范围
Hugo Enrique González-García1, Ma Teresa Santillán-Galicia1, Laura Delia Ortega-Arenas1
1Fitosanidad-Entomología y Acarología, Colegio de Postgraduados, Texcoco, Estado de Mexico, Mexico.
PeerJ
|September 17, 2025
概括
研究了麻风病毒C (CiLV-C) 和兰花斑病毒菌株 (OFV-Cit) 在墨西哥的分布. CiLV-C主要感染子,而OFV-Cit感染了,两种病毒都在无症状植物中检测到.
科学领域:
- 植物病毒学 植物病毒学
- 分子诊断学 分子诊断
- 农业昆虫学 农业昆虫学
背景情况:
- 麻风病是由麻风病毒C (CiLV-C) 和兰花斑病毒菌株 (OFV-Cit) 引起的.
- 尽管CiLV-C在2011年在墨西哥子中得到证实,但这些病毒的地理分布和宿主范围在很大程度上尚未研究.
- 了解病毒分布对于管理类麻风病至关重要,这是对类生产的重大威胁.
研究的目的:
- 在墨西哥果园中调查CiLV-C和OFV-Cit的地理分布和宿主范围.
- 为了确定感染率和确定宿主偏好 (子,,,葡萄) 对这两种病毒.
- 为了分析在石灰果园中发现的CiLV-C分离物的遗传特征.
主要方法:
- 2017年在墨西哥15个州进行了现场调查,2019年在三个州进行了现场调查,采样了子,,菜和子果园.
- 使用反转录聚合酶链反应 (RT-PCR) 试验检测出CiLV-C和OFV-Cit的存在.
- 来自果园的CiLV-C分离物的RNA2基因组区域被测序并使用BLAST进行分析以确认身份.
主要成果:
- 2017年,地理原产地显著影响了CiLV-C和OFV-Cit感染比例. 果园的CiLV-C感染率高于石灰,而石灰园的OFV-Cit感染率更高.
- 无症状的叶子对两种病毒均呈阳性,表明潜在的隐藏传播.
- 2019年,在石灰果园中检测到低感染率 (6% CiLV-C,3% OFV-Cit),测序的CiLV-C与已知的菌株相似98.99%.
结论:
- 在墨西哥果中观察到CiLV-C和OFV-Cit分布和宿主偏好的显著差异.
- 无症状植物中病毒的存在凸显了疾病检测和管理的挑战.
- 这些发现为改善现场监测策略和早期检测墨西哥类病提供了必要的数据.
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