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Updated: Jan 17, 2026

Identification of Protein Interacting Partners Using Tandem Affinity Purification
10:02

Identification of Protein Interacting Partners Using Tandem Affinity Purification

Published on: February 25, 2012

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TIE-UP-SIN:一种用于增强蛋白质-蛋白质相互作用识别的新方法

Maximilian Schedlowski1, Stephan Michalik1, Tilly Hoffmüller1

  • 1Department Functional Genomics, Center for Functional Genomics of Microbes, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, Germany.

Frontiers in microbiology
|September 17, 2025
PubMed
概括

我们开发了TIE-UP-SIN,这是一种新的方法,用于在体内识别蛋白质与蛋白质相互作用 (PPI). 这种强大的技术准确地绘制了短暂的相互作用,并量化了合作伙伴,提高了蛋白质组学研究中的可重复性.

关键词:
亲和力净化-质谱-质谱学甲交叉连接的形式化重的代谢标签重的代谢标签在体内交叉连接 in vivo交叉连接质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量蛋白质蛋白质相互作用

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