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固有微孔性聚合物 (PIMs) 与固定碳酸酶 (CA) 用于体外膜氧化

Xingci Liu¹, Lunhao Zhi¹, Chong Cheng¹

¹College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu, 610065, People's Republic of China. sunshudong@scu.edu.cn.

Journal of materials chemistry. B

|September 17, 2025

概括

这项研究将碳酸 anhydrase 固定在经过修改的 PIM-1 膜上,以增强用于体外膜氧化 (ECMO) 应用的二氧化碳去除. 固定酶显著改善了二氧化碳的清除,解决了ECMO治疗中的一个关键挑战.

科学领域:

生物材料科学生物材料科学
化学工程是化学工程的重要组成部分.
呼吸系统生理学呼吸系统生理学

背景情况:

身体外膜氧化 (ECMO) 使用聚4-甲基-1- (PMP) 膜,但它们的疏水性会导致血栓形成.
本质微孔性聚合物 (PIM) 为ECMO膜提供了优越的透性和化学修饰潜力.
之前的研究修改了PIM-1膜 (AO-PIM-1,C-PIM-1) 以提高血红相容性和气体交换,但有效的二氧化碳从血液二氧化碳中去除仍然是一个挑战.

研究的目的:

在PIM-1膜上固定碳酸酶 (CA),以增强从血液中去除二氧化碳.
研究在不同PIM-1基板上对酶固定化的最佳移植条件.
在模拟的ECMO条件下评估CA固定膜的性能.

主要方法:

使用EDC/NHS合,碳酸酶 (CA) 被固定在PIM-1膜上.
作为基质,使用了氨基胺功能化的PIM-1 (AO-PIM-1) 和氧基/氨基胺功能化的PIM-1 (C-PIM-1).
在各种移植条件和ECMO模拟下评估了酶固定效率,保留活性和二氧化碳去除.

主要成果:

氨基胺功能化的PIM-1 (AO-PIM-1) 显示出最佳的酶不动化效率 (78.35%).
在14天后,固定CA保持了高活性 (89.03%),显著超过自由CA (39.7%).
ECMO模拟测试证实,由于固定CA的催化活性,二氧化碳去除能力大大提高.

结论:

将碳酸无水酶固定在修改后的PIM-1膜上是一种有前途的策略,可以改善ECMO中的CO2去除.
AO-PIM-1基质提供了卓越的酶固定和稳定性,解决了当前膜氧化器技术的关键局限性.
这种方法为在PIM上进行酶接种提供了一个参考,用于先进的生物医学应用.

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