皮性新陈代谢的缺陷使金黄色葡萄球菌 (Staphylococcus aureus) 的小殖民地变种对二碳酸盐敏感
Asif Iqbal1, Zannatul H Tumpa1, Wyatt W Wittliff1,2
1Department of Pathobiological Sciences, Louisiana State University and Agricultural and Mechanical College, School of Veterinary Medicine, Baton Rouge, Louisiana, USA.
双碳酸通过破坏它们的缺陷的厌氧代谢来抑制金黄色葡萄球菌小殖民地变体 (SCVs),从而导致细胞质化和毒性. 这一发现表明二碳酸盐是慢性黄金色杆菌感染的潜在治疗辅助剂.
科学领域:
- 微生物学 微生物学
- 生物化学 生化学
- 传染性疾病 传染性疾病
背景情况:
- 黄金葡萄球菌表现出代谢灵活性,使其能够持久性和抗生素耐药性.
- 黄金菌的小殖民地变体 (SCV) 与持久性感染和抗生素耐受性增加有关.
- 二碳酸盐是一种内源性抗微生物药物,对S. aureus SCVs的影响尚不清楚.
研究的目的:
- 为了研究二碳酸盐对S. aureus SCVs的影响.
- 为了阐明二碳酸盐在S. aureus排毒中的皮性代谢的作用.
- 探索二碳酸盐作为一种潜在的治疗策略,以对抗慢性金黄色细菌感染.
主要方法:
- 培养S. aureus野生类型和SCVs具有不同的二碳酸盐度.
- 评估生长抑制,细胞质pH值和性代谢.
- 测量酸盐炭酸酶 (Pyc) 活性和酸盐水平.
- 评估SCV对氨基甘油酸的重新敏感性和对中性粒细胞杀死的敏感性.
主要成果:
- 二碳酸盐在不影响野生型菌株的度下抑制S. aureus SCV的生长.
- 由于酸盐水平不足,SCVs表现出缺陷的二碳酸盐性代谢,导致细胞质化和毒性.
- 外源性酸盐恢复了皮质代谢,降低了细胞质pH值,并保护SCVs免受二碳酸盐毒性.
- 双碳酸盐治疗使SCV对氨基糖化物重新敏感,并增加对杀死中性粒细胞的敏感性.
结论:
- 二碳酸的性代谢是S. aureus中毒解毒的关键机制.
- 在SCVs中缺陷的阿纳佩洛斯代谢会导致二碳酸盐敏感性和毒性.
- 调节厌敏性新陈代谢,可能与pyruvate,可以克服SCV耐药性.
- 二碳酸盐作为一种抗菌辅助剂,增强抗生素的疗效,降低金黄色细菌的毒性.
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