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针对Vibrio spp的抗菌活性Bacillus siamensis B01的基因组和功能表征

Co Thi Kim Nguyen^1,2,3, Hoang Duc Nguyen^4,5

¹Center for Bioscience and Biotechnology, University of Science, Ho Chi Minh City, Vietnam.

Current microbiology

|September 17, 2025

概括

来自越南的Bacillus siamensis B01显示出强大的抗菌活性对抗Vibrio物种. 基因组分析揭示了独特的基因和生物合成途径,突出了其作为水产养殖益生菌的潜力.

科学领域:

微生物学微生物学
基因组学就是基因组学.
生物技术是生物技术.

背景情况:

水产养殖由于致病性维菌感染而面临重大损失.
益生菌为水产养殖中的疾病控制提供了抗生素的可持续替代品.

研究的目的:

为了研究从越南红树林中分离出来的Bacillus siamensis B01的生物,基因组和抗Vibrio特性.
评估B. siamensis B01作为对抗Vibrio病原体的益生菌剂的潜力.

主要方法:

基因组比较分析,包括平均核酸标识 (ANI) 和数字DNA-DNA杂交 (dDDH).
全基因组测序和注释以识别基因,质粒和生物合成途径.
表型特征,包括生长条件,酶分泌和生物膜形成.
使用乙酸乙烯提取物对各种Vibrio物种进行抗菌活性测定.

主要成果:

西安杆菌B01与西安杆菌B. siamensis KCTC 13613T.具有很高的基因组相似性 (98.8% ANI, 97.5% dDDH).
该基因组草案包含3738个编码序列 (CDS) 和一个没有毒性或抗生素耐药性基因的等离子体.
鉴定了抗菌化合物的基因集群 (细烯,风素,辛西丁,细菌素),与已知途径有100%的相似性.
B. siamensis B01对Vibrio azureus,V. sinaloensis,V. neocaledonicus,V. alginolyticus和V. parahaemolyticus (15.7-24.3毫米的抑制区) 呈现了广泛的抗菌活性.
该菌株表现出对性pH和10%NaCl的耐受性,产生了各种酶,并形成了生物膜.

结论:

西安菌B01由于其基因组特征和显著的抗Vibrio活性,具有有前途的益生菌潜力.
这种菌株代表了一个可行的候选人,可以在水产养殖环境中开发针对Vibrio病原体的生物控制策略.

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