在Physcomitrella光生物反应器中产生人乳头瘤病毒16型病毒样颗粒
Paul Alexander Niederau1, Maria Caroline Weilguny1,2, Sarah Chamas1
1Plant Biotechnology, Faculty of Biology, University of Freiburg, Schänzlestr. 1, 79104, Freiburg, Germany.
Plant cell reports
|September 17, 2025
概括
这项研究首次证明了非血管植物,Physcomitrella的病毒样颗粒 (VLPs) 的产生. 这些在质体中产生的VLP显示出作为人类乳头瘤病毒 (HPV) 疫苗候选人的潜力.
科学领域:
- 生物技术是生物技术.
- 植物分子生物学 植物分子生物学
- 疫苗开发 疫苗开发
背景情况:
- 病毒样颗粒 (VLP) 对于子单位疫苗和药物输送至关重要,因为它们的免疫性和安全性.
- 树Physcomitrella (Physcomitrium patens) 提供了一个可扩展的,以植物为基础的表达系统,具有人性化的糖化,适合生物制药生产.
- 由L1蛋白组成的人类乳头瘤病毒 (HPV) VLP,是预防HPV相关癌症的关键.
研究的目的:
- 建立一种新的基于植物的系统,用于生产人类乳头瘤病毒 (HPV) 16种类似病毒的颗粒 (VLP).
- 通过将L1蛋白向摩Physcomitrella中的叶绿体来优化VLP的生产.
- 开发一种净化和重组协议,用于从生物质中产生功能性HPV-16 VLP.
主要方法:
- 利用菲斯科米特雷拉 (Physcomitrella) хлоропласт过境来引导L1蛋白表达到 хлоропласт中.
- 采用共聚焦激光扫描显微镜来确认L1蛋白的亚细胞定位.
- 开发了一种涉及硫酸沉和阴离子交换色谱的净化策略.
- 通过对净化L1蛋白的受控解离和再结合,实现了VLP组装.
主要成果:
- 通过向叶绿体,实现了重组L1蛋白的高产量,超过每克新鲜体重0.3毫克.
- 从生物质中成功净化了L1蛋白.
- 证明了将纯化的L1蛋白重新组装成完全形成的HPV-16 VLP的能力.
- 首次证实了非血管植物系统中VLP的生产.
结论:
- 菲斯科米特雷拉是生产,净化和组装病毒样颗粒 (VLP) 的可行和高效平台.
- 叶绿体向显著提高了基于的表达系统中的重组蛋白质产量.
- 这项工作提出了一种新的,可扩展的方法,用于生成VLP疫苗候选者,特别是针对HPV,使用非血管植物.
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