通过光化学内部化,通过超微型光核-的基酸纳米粒子进行增强的细胞内传递
Nada Naguib1,2, Jacob A Erstling1,2, James F Tallman1
1Department of Materials Science and Engineering, Cornell University, Ithaca, New York 14853, United States.
ACS biomaterials science & engineering
|September 17, 2025
概括
甲蓝功能化纳米颗粒克服了内体捕获,以有效地提供细胞质. 超小的核心外颗粒使控制的光化学内化和核结构的访问,推进纳米医学.
科学领域:
- 纳米技术 纳米技术
- 生物医学工程 生物医学工程
- 摄影化学的使用.
背景情况:
- 纳米粒子药物输送受到内体捕获的阻碍,限制了细胞体释放和细胞内向.
- 甲蓝 (MB) 和3 (Cy3) 分别是光敏化剂和光剂,在光动力学疗法和成像中具有潜力.
研究的目的:
- 开发超小的,甲蓝功能化的酸纳米颗粒,通过光化学内化 (PCI) 进行高效的细胞内输送.
- 研究链接器长度对纳米粒子光物理性质和细胞质递送效率的影响.
- 为了证明受控亚细胞局部化和顺序货物交付的潜力.
主要方法:
- 合成超小 (4-5 nm) 核心外的酸纳米颗粒封装Cy3并通过短 (PEG4) 或长 (PEG14) 链接器与MB功能化.
- 光物理特征,包括单点氧量子产量测量.
- 使用活细胞成像和共聚焦显微镜对HeLa细胞进行细胞吸收和细胞内贩运研究.
- 为PCI优化红灯照明参数.
主要成果:
- 与短链MB-PEG4-Cy3-aC'dots相比,长链MB-PEG14-Cy3-aC'dots表现出优异的细胞质输送,尽管单片氧量子产量较低.
- 优化的PCI协议 (15分钟红光) 实现了从内体的扩散细胞质分布,细胞存活率达80%.
- 超微小的纳米颗粒有效地转移到细胞核,表明进入受限制的细胞内隔间.
- PCI治疗促进了二次纳米颗粒的顺序递送,表明内体融合和膜透.
结论:
- 纳米颗粒的设计,特别是链接器的长度,对PCI的细胞质递送效率产生重大影响.
- 超小的MB功能化纳米粒子为受控的细胞内向提供了一个多功能平台,并有可能进行多重货物交付.
- 这种方法克服了内分体捕获,为提高精度的纳米医学应用铺平了道路.
相关概念视频
01:37Super-resolution Fluorescence Microscopy
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
01:25Overview of Electron Microscopy
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.