分子特征,抗微生物耐药性分析和从死在中的胚胎卵中分离的沙门氏菌的生物膜分析
Swagat Khanal1, Himal Luitel2, Sujan Adhikari1
1Faculty of Animal Science, Veterinary Science and Fisheries, Agriculture and Forestry University, Rampur, Chitwan, Nepal.
Poultry science
|September 17, 2025
概括
在死在中的蛋中发现了多药耐药的沙门氏菌,对常见的抗生素具有很高的耐药性,并形成生物膜. 这对公共卫生构成重大风险,需要改善化场生物安全和抗菌药物管理.
科学领域:
- 兽医微生物学 兽医微生物学
- 食品安全 食品安全
- 分子流行病学分子流行病学
背景情况:
- 在家禽化场的沙门氏菌污染对食品安全和动物健康构成重大威胁.
- 死在中的胚胎卵作为在家禽生产系统内传播病原性细菌的潜在储备.
研究的目的:
- 从死蛋中分离出沙门氏菌的特征,重点关注抗菌素耐药性 (AMR),生物膜形成和毒性基因.
- 评估多药耐药 (MDR) 沙门氏菌在家禽生产中的公共卫生影响.
主要方法:
- 使用培养,生化和分子技术 (PCR) 分析了210个蛋样本.
- 抗微生物敏感性测试和生物膜形成测试.
- 检测主要的沙门氏菌毒性基因 (invA,spvC,spvB1) 和生物膜相关基因 (agfA).
主要成果:
- 沙门氏菌的发病率为11.42%,对塞法列辛 (100%),安皮西林 (95.83%) 和纳利迪克酸 (91.67%) 的耐药性很高.
- 83.33%的分离物是多重耐药 (MDR),平均多重抗生素耐药性指数为0.51.
- 70.83%的分离物表现出产生生物膜的能力,并确定了病毒性基因 invA,spvC,spvB1 和 agfA.
结论:
- 有生物膜形成潜力的MDR沙门氏菌株在化场中普遍存在,对食物链构成重大风险.
- 加强生物安全,明智使用抗微生物药物和定期监测对于控制家禽中的沙门氏菌感染至关重要.
- 调查结果强调需要综合战略,以减轻沙门氏菌在家禽生产中的经济和健康影响.
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