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埃舍里希亚大肠杆菌的代谢工程用于高效的N-乙神经胺酸生产

Chao Sun¹, Jinhang Yi¹, Ying Zhang¹

¹College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.

Bioresource technology

|September 17, 2025

概括

为了改善大肠杆菌中的N-乙神经胺酸 (Neu5Ac) 生产,研究人员优化了代谢途径,以减少乙副产品的积累. 这导致了创纪录的Neu5Ac产量,提高了其对工业应用的潜力.

科学领域:

生物技术是生物技术.
代谢工程是代谢工程.
微生物发酵微生物发酵

背景情况:

大肠杆菌中的乙酸溢出阻碍了细胞生长和生物合成,特别是对于需要酸 (PEP) 的N-乙神经胺酸 (Neu5Ac) 等产品.
高酸盐水平显著限制了大肠杆菌细胞工厂生产有价值化学品的效率和可扩展性.

研究的目的:

系统地优化PEP分布,减少乙酸积累,以实现高效的Neu5Ac生物合成.
制定战略,以提高使用大肠杆菌的增值化学品的生产.

主要方法:

应用了代谢工程策略,以准大肠杆菌中的乙酸合成途径.
优化了发酵温度和ATP供应,以提高PEP利用率并减少副产品的形成.
用N-乙神经胺酸 (Neu5Ac) 的生物合成作为模型系统.

主要成果:

开发了一种新型菌株NEA-27,可显著减少和延迟酸盐积累.
这种菌株在56小时内在5升生物反应器中实现了77.12g/LNeu5Ac的创纪录的新产量.
该生产实现了0.217g/g葡萄糖的产量和1.38g/L/h的生产率.

结论:

该研究提出了有效的策略,用于管理PEP分布,并减轻大肠杆菌中的酸盐溢出.
开发的方法为利用PEP作为化学品工业生物合成的代谢前体提供了关键的见解.
新5Ac生产的高效率和产量显示出大规模工业应用的巨大潜力.

关键词:

乙酸乙酸盐的使用方法埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.N-乙神经氨基酸是什么?基酸是一种基酸.酸盐酶酶的使用方法

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