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Updated: Jan 17, 2026

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Hierarchical and Programmable One-Pot Oligosaccharide Synthesis
Published on: September 6, 2019
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通过双酶级联从糖和糖中联合生产2-O-α-d-Glucosyl糖醇和D-Allulose
Jiawen Li1, Yin Wang1, Haibo Hu1
1State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, P.R. China.
Journal of agricultural and food chemistry
|September 17, 2025
概括
这项研究介绍了一种双酶系统,可以从简单的基质有效地生产保湿剂 (2-O-α-d-glucosyl glycerol) 和低热量甜味剂 (D-allulose). 优化的酶平台提供了一种可持续且具有成本效益的生物生产解决方案.
科学领域:
- 工业生物技术 工业生物技术
- 酶式生物催化剂酶式生物催化剂
- 可持续化学 可持续化学
背景情况:
- 在工业生物技术中,高价值产品的低成本基质的高效生物转化仍然是一个挑战.
- 对2-O-α-d-glucosyl glycerol (2-αGG) 等保湿剂和 D-allulose 等低热量甜味剂的需求日益增加,需要创新的生产方法.
研究的目的:
- 开发一种双酶,单级联系统,用于从糖和甘油中共同生产2-αGG和D-.
- 通过蛋白质工程和优化反应条件来提高系统的效率和稳定性.
主要方法:
- 在一个单系统中,将糖酸酶 (SPase) 和D-素-3-聚酶 (DAEase) 集成.
- 包括双酶共表达,序列反应和蛋白质工程 (通过半理性设计在DAEase中的I66V突变) 的策略.
- 分子建模用于指导蛋白质工程,以改善催化活性和热稳定性.
主要成果:
- 获得的高标位:215 g/L为2-αGG和104.6 g/L为D-素.
- 证明了高的转化率:97.7%的糖糖,42.3%的甘油和60.0%的D-果糖.
- 改造的DAEase (I66V突变) 显示了增强的催化活性和热稳定性.
结论:
- 开发了一个强大的和经济的酶基平台,用于可持续的生物生产.
- 该系统可以有效地共同生产食品和化品行业的有价值化合物.
- 蛋白质工程和级联系统设计是增强生物催化过程的有效策略.
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