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一个单细胞大米地图集成了多个物种的数据,揭示了 cis-regulatory 进化

Haidong Yan^1,2, John P Mendieta³, Xuan Zhang³

¹Department of Genetics, University of Georgia, Athens, GA, USA. yanhaidong1991@163.com.

|September 17, 2025

概括

植物中调节元件 (CREs) 显示了细胞类型特定的进化. 研究人员绘制了大米和其他草的染色质可访问性,发现了叶子表皮的加速进化,并确定了类似沉声器的CREs.

科学领域:

植物基因组学植物基因组学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
进化生物学是进化的生物学.

背景情况:

控制基因表达的Cis-regulatory元素 (CREs),但他们的植物进化是不太了解.
了解CREs对于作物改良和植物基础科学至关重要.

研究的目的:

为米 (Oryza sativa) 构建一个单细胞色素可访问性地图.
研究不同植物细胞类型和物种中CREs的进化动态.
确定新的监管要素及其功能.

主要方法:

单细胞ATAC-seq在103,911个米细胞核中的126个米细胞状态.
用57,552个核来自四种草种 (玉米,,小米,信号草) 的比较基因组学.
对染色质可访问性保护和基因素修饰 (H3K27me3) 重叠的分析.

主要成果:

染色质可访问性保护是细胞类型的特异性,叶子表皮表现出米的加速进化.
保存的可访问区域重叠H3K27me3作为沉声器类CREs,抑制基因表达.
这些区域的删除导致了基因上调,证实了它们的功能.

结论:

这项研究为大米研究提供了宝贵的表观基因组资源.
对比基因组学揭示了植物CREs的动态,细胞类型特定的进化.
已识别的类似声器的CREs为植物基因调控研究提供了新的目标.

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