Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

11.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.8K
Sanger Sequencing01:57

Sanger Sequencing

773.3K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
773.3K
Next-generation Sequencing03:00

Next-generation Sequencing

97.8K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
97.8K
Ribosome Profiling02:24

Ribosome Profiling

4.1K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.1K
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

7.2K
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
7.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Concept mask-aware pruning and augmentation for few sample model compression.

Neural networks : the official journal of the International Neural Network Society·2026
Same author

Physics-Informed Optimization for the Sub-Feature-Scale Fabrication of Hollow Microneedles via Digital Light Processing.

Micromachines·2026
Same author

Crystal engineering of manganese nitride thin film electrodes for all-nitride asymmetric supercapacitors.

Chemical communications (Cambridge, England)·2026
Same author

Altered Properties of Bone Marrow-Derived Mesenchymal Stem Cells Exposed to Serum from Patients with Acute-On-Chronic Liver Failure.

International journal of stem cells·2026
Same author

An encyclopedic regulatory and functional atlas of RNA interactomes.

Nature methods·2026
Same author

Author Correction: RIP-PEN-seq identifies a class of kink-turn RNAs as splicing regulators.

Nature biotechnology·2026

相关实验视频

Updated: Jan 17, 2026

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

1.3K

NAP-seq用于全长非封顶RNA测序.

Shurong Liu1, Junhong Huang1, Lianghu Qu2

  • 1MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Pharmaceutical Functional Genes, Innovation Center for Evolutionary Synthetic Biology, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.

Nature protocols
|September 17, 2025
PubMed
概括
此摘要是机器生成的。

非封闭RNAs (napRNAs) 是丰富的,但很难研究. NAP-seq是一种测序全长napRNA的新方法,有助于发现新型调节RNA和RNA生物发生路径.

更多相关视频

Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs
08:49

Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs

Published on: September 16, 2019

8.1K
3' End Sequencing Library Preparation with A-seq2
12:01

3' End Sequencing Library Preparation with A-seq2

Published on: October 10, 2017

11.0K

相关实验视频

Last Updated: Jan 17, 2026

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

1.3K
Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs
08:49

Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs

Published on: September 16, 2019

8.1K
3' End Sequencing Library Preparation with A-seq2
12:01

3' End Sequencing Library Preparation with A-seq2

Published on: October 10, 2017

11.0K

科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 生物化学 生物化学

背景情况:

  • 哺乳动物基因组广泛转录非封闭RNA (napRNAs).
  • napRNAs调节生物过程和RNA生物发生,但由于它们的异质性,很难识别.
  • 现有的方法与napRNA的不同长度,修改和结构作斗争.

研究的目的:

  • 介绍napRNA测序 (NAP-seq) 技术的原理和详细程序.
  • 为了能够在各种细胞类型中全面识别全长的napRNA.
  • 克服napRNA发现和表征方面的挑战.

主要方法:

  • NAP-seq使用T4多核酸激酶预处理来标准化RNA终结.
  • 尺寸选择和RNase H枯竭丰富了长,低丰富度的RNA.
  • 具有随机条形码的自定义适配器,双平台测序 (牛津纳米孔和Illumina) 和优化的cDNA合成确保单核酸分辨率并最大限度地减少偏差.

主要成果:

  • NAP-seq允许在单核酸分辨率下识别具有各种终端修饰的全长napRNA.
  • 这种方法有效地丰富了低丰度,长RNAs.
  • 整个工作流,从图书馆准备到分析,可以在8天内完成.

结论:

  • NAP-seq为发现具有调节作用的新型非编码RNA提供了强大的方法.
  • 这种技术有助于在各种生物环境中研究RNA生物发生.
  • NAP-seq克服了之前在napRNA识别和表征方面的局限性.