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综合的进化,差异表达和VIGS分析揭示了GhNST1在调节高地棉花干旱耐受性和早期成熟方面的功能

Xueli Zhang¹, Dandan Li¹, Xuefeng Guo¹

¹State Key Laboratory of Aridland Crop Science, College of Life Science and Technology, Gansu Agricultural University, Lanzhou, 730070, China.

Functional & integrative genomics

|September 17, 2025

概括

NAC二次壁厚化促进因子1 (NST1) 对棉花至关重要.

科学领域:

植物生物学植物生物学
分子遗传学分子遗传学
农业科学农业科学

背景情况:

NAC二次壁加厚促进因子1 (NST1) 在植物应激反应和发育中起着关键作用.
关于高地棉花 (Gossypium hirsutum) 中GhNST1的特定功能存在有限的研究.
了解 GhNST1 对于提高棉花的弹性和产量至关重要.

研究的目的:

研究高地棉花中GhNST1的功能,特别是其对干旱耐受性和植物成熟度的作用.
在各种非生物压力下分析 GhNST1 的转录反应.
探索与开花和花发育相关的 GhNST1 的遗传调节.

主要方法:

分析 GhNST1 促销者 cis 元素.
在各种非生物性压力下 (干旱,热,NaCl,寒冷) 进行基因表达分析.
病毒诱导的基因沉默 (VIGS) 和基于棉花叶卷曲病毒 (CLCrV) 的基因沉默来评估 GhNST1 的功能.
生理和生化分析 (RWC,WLR,叶绿素,酶活动,MDA,红素,素) 和对应激和开花基因的基因表达概况.

主要成果:

GhNST1表达最强烈地受到干旱压力的诱导,在早期成熟的棉花品种中更高.
沉默GhNST1显著降低干旱耐受性,导致枯增加,相对含水量降低,生理参数发生变化.
GhNST1沉默还通过降低促进开花的基因 (GhAP1,GhFT,GhSOC1) 和提高抑制剂 (GhSVP) 的调节来延迟开花和花发育.

结论:

GhNST1对于高地棉花的干旱耐受性至关重要.
GhNST1具有双重作用,调节非生物应激反应和过渡到生殖发育 (早期成熟) 的过程.
这些发现提供了对提高棉花抗旱能力和优化作物成熟度的遗传策略的见解.

关键词:

在 GhNST1 找 GhNST1 干旱造成的压力是干旱.提前到期的提前到期期限高地棉花是高地的棉花.病毒诱导的基因沉默 (VIGS)

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