使用转录和翻译调节剂来改善CHO细胞中的重组蛋白表达
Shaolei Geng1,2,3, Chunliu Mi1,2, Chong Wang1,4
1International Joint Research Laboratory for Recombinant Pharmaceutical Protein Expression System of Henan, Xinxiang Medical University, Xinxiang 453003, China.
Acta biochimica et biophysica Sinica
|September 18, 2025
概括
本综述探讨了通过改造转录因子 (TF) 和翻译调节剂来增强中国仓鼠卵巢 (CHO) 细胞中的复合治疗蛋白 (RTP) 生产. 基因组学和合成生物学方面的进步为优化CHO细胞系提供了新的策略.
科学领域:
- 生物技术是生物技术.
- 细胞生物学 细胞生物学
- 蛋白质工程是指蛋白质工程.
背景情况:
- 中国仓鼠卵巢 (CHO) 细胞是生产重组治疗性蛋白质 (RTP) 的主要系统.
- 提高RTP产量,质量和降低成本是行业的关键目标.
- 转录和翻译是CHO细胞中RTP生成的关键过程.
研究的目的:
- 通过转录因子 (TF) 和翻译调节剂,审查增强CHO细胞中RTP表达的策略.
- 探索TF工程在改善生长,新陈代谢和ER功能中的作用.
- 要突出翻译调节剂对蛋白质折叠,分泌和免疫性的影响.
主要方法:
- 对CHO细胞中TF和翻译调节器工程现有文献的审查.
- 对TF和转化因子设计的基因组学和合成生物学进步的分析.
- 总结了优化RTP表达和CHO细胞系发展的策略.
主要成果:
- TF工程是通过定制TF和推广器来增强RTP表达的有效策略.
- 翻译调节者涉及许多基因和途径,对于蛋白质折叠,分泌和免疫性至关重要.
- 基因组学和合成生物学可以设计特定的TF和选择转化因子.
结论:
- 工程转录因子和翻译调节器可以显著增加CHO细胞中的RTP表达.
- 利用基因组学和合成生物学进步可以导致更高效的CHO细胞系和优化的蛋白质表达.
- 进一步的优化策略是提议的,以提高RTP的生产和降低制造成本.
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