分析变异对实证生物学变异的影响-在兽医临床病理学实验室的分析性能规范中得出的分析性能规格
A Russell Moore1, Addison Adams2
1Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA.
Veterinary clinical pathology
|September 18, 2025
概括
兽医实验室分析性能规范 (APS) 的经验生物变异 (EBV) 方法对于表现不佳的实验室可能是宽容的. 由于不同性能水平的结果不一致,其适用于兽医的适用性需要进一步评估.
科学领域:
- 兽医临床病理学 兽医临床病理学
- 分析化学 分析化学
- 生物统计学 生物统计学
背景情况:
- 实证生物变异 (EBV) 方法用于导出分析性能规范 (APSs) 正在对兽医临床病理学进行评估.
- 分析性能对EBV APS的影响在整个兽医实验室性能谱中还没有得到很好的定义.
研究的目的:
- 在兽医临床病理学中典型的各种分析变化系数 (CVA) 中建模和比较EBV APS.
- 评估EBV APS与允许总误差 (TEA) 和传统生物变异 (BV) 模型相对的一致性.
主要方法:
- 使用已发表的兽医生物化学测量和CVA数据以及狗参考间隔 (RI).
- 使用蒙特卡洛模拟来建模EBV APSs.
- 将模拟的EBV APS与来自TEA和传统BV模型的EBV APS进行了比较.
主要成果:
- 由于分析性能差,EBV衍生的允许CVA (pCVA) 通常小于观察到的CVA.
- EBV APS与TEA和BV APS的关系不一致,特别是当分析性能被认为是可以接受的时.
- 无法接受的分析性能导致EBV APS大幅增加,失去与TEA/BV模型的关系.
- 对于高斯数据的日志正常计算导致了更广泛的EBV APSs.
结论:
- 该EBV方法产生以增长为导向的APS,可能为表现不佳的兽医实验室提供更多宽容.
- 由于可变的APS和与已确定的错误指标的不一致关系,EBV方法对兽医的适用性是可疑的.
- 需要进一步调查,以确定EBV方法在兽医临床病理学中的适用性.
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