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Fabian Schubert¹, Elsa Arcalís¹, Maximilian Kyral¹

¹Institute of Plant Biotechnology and Cell Biology, Department of Biotechnology and Food Sciences, BOKU University, Vienna, Austria.

Frontiers in plant science

|September 18, 2025

概括

植物性蛋白质体 (PBs) 被设计成封装鱼类过敏原,增强它们对消化的稳定性. 这种新的生物封装策略显示出改善口服免疫疗法治疗的前景.

科学领域:

植物生物技术植物生物技术
过敏研究研究过敏研究
生物材料科学是生物材料的科学.

背景情况:

口服免疫疗法 (OIT) 对过敏有效,但过敏原在肠道中面临消化.
开发稳定的过敏原输送系统对于OIT的有效性至关重要.
基于植物的系统为控制过敏原释放和保护提供了潜力.

研究的目的:

在 *Nicotiana benthamiana* 中设计和表征多层蛋白体 (PBs),用于封装鱼类过敏原parvalbumin.
为了评估封装过敏原的稳定性与模拟的胃肠道消化.
评估PBs作为口服免疫治疗的输送载体的潜力.

主要方法:

在 *Nicotiana benthamiana* 中共同表达这些变体以创建多层 PB.
对焦显微镜和相关光电子显微镜 (CLEM) 用于结构分析.
在体外消化试验和Caco-2细胞吸收研究以模拟胃肠道状况.

主要成果:

成功生成了含有帕瓦胺衍生物的多层PBs,具有独特的核心外结构.
与可溶性过敏原相比,封装过敏原对模拟的胃肠道消化有明显的增强,层特异性抗性.
展示了人类肠道上皮细胞对PB吸收的潜力.

结论:

设计的多层PB为过敏原封装提供了保护平台,增强了对蛋白质分解消化的抵抗力.
这种生物封装策略为开发改进的口服免疫治疗输送工具提供了一种新的方法.
该研究促进了对蛋白质储存器官形成和生物封装的理解,用于治疗应用.

关键词:

过敏原是一种过敏原.生物封装是一种生物封装.分子农业是分子农业.植物性生产是以植物为基础的.再组合药物制品的使用.储存器官储存器官储存器官

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