可扩展的单细胞总RNA-seq揭示了免疫,感染和大脑发育中的非编码程序.
Alina Isakova1, Stephen Quake1, Daniel Liu2
1Stanford University.
Research square
|September 18, 2025
概括
这项研究扩展了单细胞RNA测序以捕获多种不同的非编码RNA,揭示了它们在细胞身份和病毒感染中的作用. 发现包括免疫细胞,大脑和登革热病毒感染期间的非编码RNA程序.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 非编码RNAs (ncRNAs) 对于转录后调节至关重要,但通常会被标准单细胞RNA测序 (scRNA-seq) 遗漏.
- 现有的scRNA-seq方法主要捕获多基化转录,限制了对短或非多基化ncRNA的研究.
研究的目的:
- 调整scRNA-seq以全面捕获编码和非编码RNA,包括短和非多基化物种.
- 探索ncRNAs在不同生物环境中的细胞身份和状态中的功能性作用.
主要方法:
- 修改了10x Genomics scRNA-seq平台,以捕获更广泛的RNA生物型.
- 将该方法应用于免疫细胞,病毒感染的肝细胞和正在发育的人类大脑组织.
- 分析了已识别的ncRNA的表达模式和调节关系.
主要成果:
- 在单细胞分辨率下成功捕获了各种ncRNAs (miRNAs,tRNAs,lncRNAs,histonRNAs,病毒转录).
- 在免疫细胞,感染肝细胞和发育中的大脑中确定了动态的ncRNA程序.
- 在大脑中发现了细胞类型特定的ncRNA,包括Cajal-Retzius细胞中的MIR137,与神经发育障碍有关.
结论:
- 在scRNA-seq中扩展的转录组覆盖面揭示了ncRNAs对基因调节的关键层.
- ncRNAs在定义细胞身份,状态和指导发育过程方面发挥着重要作用.
- 这种方法提供了一种可扩展的方法,用于单细胞中全面的ncRNA分析.
更多相关视频
06:38Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing
Published on: October 12, 2018
19.6K
07:49Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing DIVA-Seq
Published on: October 26, 2018
9.8K
相关概念视频
03:21RNA-seq
11.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.8K
02:24Ribosome Profiling
4.1K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.1K
02:15Experimental RNAi
7.3K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
7.3K
02:28Leaky Scanning
5.6K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.6K