在肝硬化小鼠模型中,微生物群的组成和肠道屏障功能受到tamsulosin和Lactococcus lactis的调节
Silvia Valeria Padilla-García1, Abraham Loera-Muro2, Martín Humberto Muñoz-Ortega3
1Department of Morphology, Center for Basic Sciences, Autonomous University of Aguascalientes, Aguascalientes, Ags, Mexico.
Frontiers in cellular and infection microbiology
|September 18, 2025
概括
在大鼠肝硬化模型中,与Lactococcus lactis和tamsulosin同时治疗改善了肠道微生物群平衡和肠道屏障功能. 这种组合疗法减少了细菌转移,改善了肠道健康.
科学领域:
- 胃肠病学和肝病学
- 微生物学 微生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 肝硬化逐渐破坏肠肝轴.
- 乳球菌 (L. lactis) 增强了肠道屏障功能,并具有免疫调节作用.
- 在肝损伤模型中,tamsulosin表现出抗纤维素和抗炎性质.
研究的目的:
- 研究塔姆苏洛辛和L. lactis对肠道微生物群恢复和肠道屏障完整性的联合作用.
- 评估这种联合治疗在肝硬化Wistar大鼠模型中的疗效.
主要方法:
- 在使用CCl4.4的Wistar大鼠中诱导肝硬化.
- 鼠被用tamsulosin,L. lactis或组合疗法治疗.
- 肠道微生物组成通过16SrRNA测序来分析.
- 通过测量紧结蛋白表达 (ZO-1,ocludin,claudin-2) 和评估细菌转位来评估肠道屏障完整性.
主要成果:
- 同时使用L. lactis和tamsulosin进行治疗 (T/L. 乳糖) 增加了微生物多样性,并促进了平衡的肠道微生物群.
- 乳糖治疗上调了ZO-1和奥克卢丁的表达,改善了肠道形态.
- 细菌转移显著减少,在肠外组织中没有检测到内毒素或细菌.
结论:
- 坦素和L. lactis,特别是结合在一起,对肝硬化中肠道屏障产生积极影响.
- 联合治疗显示出恢复肠道微生物群平衡和完整的潜力.
- 减少细菌转移和改善肠道形态突出了这种综合方法的治疗益处.
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