Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

599
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
599
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

517
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
517
Methods of Classification and Identification01:28

Methods of Classification and Identification

1.0K
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
1.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

A syntenic pangenome of Gardnerella reveals novel plasmids and phage, taxonomic boundaries, and species-level stratification of metabolic and virulence potential.

Nature communications·2026
Same author

Exposure to Antibiotics Modifies the Immune Profiles of Bacterial Extracellular Vesicles from Common Vaginal Anaerobes.

bioRxiv : the preprint server for biology·2026
Same author

A microphysiologic human cervical model recapitulates microbial, immune, and pathogenic properties of sexually transmitted infections.

Science advances·2026
Same author

Vaginal microbiota impacts of a Lactobacillus crispatus live biotherapeutic and predictors of colonization in randomized controlled trial.

Cell host & microbe·2026
Same author

Pathological phimosis is associated with foreskin immune cell infiltration but not microbiota composition.

mSphere·2026
Same author

Faecal microbiota transplant for chronic norovirus infection: a case report of donor microbiota engraftment without clinical success.

Gastroenterology report·2026

相关实验视频

Updated: Jan 17, 2026

Precise, High-throughput Analysis of Bacterial Growth
09:00

Precise, High-throughput Analysis of Bacterial Growth

Published on: September 19, 2017

24.8K

kSanity:基于k-mer的应用程序,用于精确检测和定量细菌菌株.

Michael France1,2, Issac Chaudry1,3, Joseph Elsherbini4

  • 1Center for Advanced Microbiome Research and Innovation, Institute for Genome Sciences, University of Maryland, School of Medicine, Maryland, USA.

bioRxiv : the preprint server for biology
|September 18, 2025
PubMed
概括

在临床样本中精确的细菌菌株量化是至关重要的. 基于k-mer的新工具kSanity精确地检测和量化元基因组数据中的向细菌菌株,克服类似菌株的干扰.

关键词:
细菌菌株检测检测的方法转基因组学是指转基因组学.

更多相关视频

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
06:34

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Published on: July 11, 2016

19.9K
Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
11:09

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

16.7K

相关实验视频

Last Updated: Jan 17, 2026

Precise, High-throughput Analysis of Bacterial Growth
09:00

Precise, High-throughput Analysis of Bacterial Growth

Published on: September 19, 2017

24.8K
Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
06:34

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Published on: July 11, 2016

19.9K
Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
11:09

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

16.7K

科学领域:

  • 微生物学 微生物学
  • 生物信息学是一种生物信息学.
  • 基因组学就是基因组学.

背景情况:

  • 准确的细菌菌株检测和量化对于了解临床样本中的殖民和持久性至关重要.
  • 现有的方法,如qPCR和枪甲基因组读取映射,容易受到居住微生物群体内的类似细菌菌株的干扰.

研究的目的:

  • 介绍kSanity,一种基于k-mer的新型应用程序,旨在精确检测和量化 shotgun metagenomic 数据中的特定细菌菌株.
  • 通过减少同类菌株的干扰来解决当前方法的局限性.

主要方法:

  • kSanity使用基于k-mer的分析,对测序读取和参考基因组进行精确的字符串匹配.
  • 使用in silico spike-in实验和in vivo观察数据来评估性能.

主要成果:

  • kSanity展示了针对细菌菌株的精确和准确量化.
  • 该应用程序即使在基因组内具有低序列覆盖率的目标菌株存在时也有效.
  • kSanity显示与传统方法相比,对同类菌株干扰的敏感性降低.

结论:

  • 在复杂的元基因组样本中,kSanity为细菌菌株量化提供了强大的解决方案.
  • 该工具提高了分析微生物群落的准确性,特别是在临床环境中.
  • kSanity可以作为一个开源应用程序用于更广泛的研究使用.