功能上是必不可少的,结构上是多样化的:通过光遗传IFT88扰乱和体积电子显微镜洞察斑马鱼左右组织者
Favour Ononiwu1,2, Melissa Mikolaj3,4, Christopher Dell3,4
1Department of Biology, Syracuse University, USA.
bioRxiv : the preprint server for biology
|September 18, 2025
概括
毛是斑马鱼Kupffer's Vesicle (KV) 发育和光形成的重要组成部分. 结构分析揭示了多种不同的KV子子类型,为左右轴规格提供了新的见解.
科学领域:
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
- 遗传学 是一个遗传学.
背景情况:
- 斑马鱼中的Kupffer's Vesicle (KV) 作为左右组织器 (LRO).
- 在KV内的乳对其功能至关重要,但它们的结构多样性和在形态发生过程中的作用尚未完全理解.
- 了解KV的结构是解读左右不对称性决定的关键.
研究的目的:
- 研究在KV形态发生过程中的作用.
- 描述斑马鱼KV内的结构多样性和超结构.
- 探索KV cilia的潜在亚型和空间组织.
主要方法:
- 设计了一种转基因斑马鱼 (sox17:Cry2-GFP) 线,用于光遗传学上破坏内运输蛋白IFT88.8.
- 利用光遗传学来扰乱KV祖先的纤毛发育.
- 使用体积电子显微镜 (vEM) 进行高分辨率的3D超结构绘制KV毛.
主要成果:
- IFT88的光遗传性干扰损害了纤毛发育和KV光的形成,证实了纤毛在形态发育中的作用.
- vEM揭示了KV cilia中显著的结构异质性,只有70.1%保留了两个中心.
- 详细的分析显示,中心叶附属体和根叶纤维的百分比不同,以及空间偏向的纤维相关囊泡 (CaVs) 和密集囊泡 (CaDVs).
结论:
- 乳毛是KV发育和光形成不可或缺的.
- 斑马鱼的KV毛表现出了显著的结构多样性和空间图案.
- 这些发现突出了LRO组织和状细胞专业化中一个新的复杂性层,用于左右轴的确定.
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