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Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

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Updated: Apr 29, 2026

Lensless Fluorescent Microscopy on a Chip

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

无面具和芯片上的LED阵列显微镜,具有空间可变角度校准,用于厘米尺度相位成像.

Sibi Chakravarthy Shanmugavel¹, Vindya Senanayake², Donghwa Suh²

¹Chandra Department of Electrical and Computer Engineering, University of Texas at Austin, Austin, TX, USA.

bioRxiv : the preprint server for biology

|September 18, 2025

概括

本研究介绍了一种无面具的计算方法,用于使用LED阵列进行大视野 (FOV) 相位成像. 它克服了双图像模糊性和不同的照明角度,以实现生物组织的高分辨率,经济高效的成像.

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Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

相关实验视频

Last Updated: Apr 29, 2026

Lensless Fluorescent Microscopy on a Chip

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

科学领域:

光学是什么?光学是什么?光学是什么?
生物医学成像技术生物医学成像技术
计算成像技术的成像

背景情况:

基于LED阵列的角度照明使成本高效,大视野 (FOV) 阶段成像成为可能.
挑战包括双图像模糊性降低相位重建和空间变化的照明角度影响大FOV精度.
现有的基于面具的解决方案增加了系统的复杂性.

研究的目的:

开发用于无面具芯片相位成像的计算框架.
为了实现空间变化的LED照明角度的自适应校准.
为了使生物样品的高质量,大FOV相位成像.

主要方法:

将传感器FOV划分为具有近似平面LED照明的子区域.
从几何上初始化LED照明角度,并在相检索过程中对其进行精细化.
在每个子区域内进行相位重建之前,使用软光学透明度.
将重建的相位图合并为一个完整的大FOV图像.

主要成果:

展示了厘米尺度的芯片上相位成像 (高达2.7×1.7厘米2).
在各种生物组织部分实现了微米级分辨率.
成功完成了无面具相位成像和自适应照明角度校准.

结论:

计算框架有效地解决了双图像模糊性和照明角度变化.
无需复杂的光学面具,可实现高效,高分辨率,大FOV相位成像.
在芯片上对生物样本进行相位成像方面取得了重大进展.