在microRNA集群辅助期间,微处理器及其辅助因子ERH和SAFB1/2的可分离作用
Renfu Shang1, Niko Popitsch2,3, Seungjae Lee1
1Developmental Biology Program, Sloan Kettering Institute, 430 East 67th St, ROC-10, New York, NY 10065, USA.
bioRxiv : the preprint server for biology
|September 18, 2025
概括
微型RNA集群辅助通过微处理器复合体转移增强了亚最佳的miRNA生物发生. ERH促进了转移,而SAFB因子稳定了结合,揭示了聚类微RNA的调节途径.
科学领域:
- 分子生物学分子生物学
- 在RNA生物学,RNA生物学.
- 基因规则 基因规则
背景情况:
- 微RNAs (miRNAs) 是关键的小RNAs,可以调节基因表达.
- 一些miRNA位点具有低于最佳的特征,需要有效生物发生的调节机制.
- 集群辅助,其中一个最佳miRNA邻居帮助一个次优的邻居,是一个已知的但不太了解的过程.
研究的目的:
- 阐明 miRNA 集群协助背后的机制.
- 确定微处理器复合体及其在这个过程中的辅助因子之间的关键相互作用.
- 确定ERH和SAFB因子在miRNA集群援助中的作用.
主要方法:
- 使用了微处理器复合体和辅助因子的突变细胞 (Drosha,DGCR8,ERH,SAFB1/2).
- 分析了miRNA结构-功能变体,并采用了生物化学测试.
- 进行全基因组分析和表观性测试,以排序辅因子功能.
主要成果:
- 根据集群辅助,定义了亚最佳miRNAs的特征.
- 确定了微处理器-辅助因子相互作用的子集,对集群辅助至关重要.
- 建立了一个途径,其中ERH调解微处理器传输,SAFB因子 (SAFB2) 调解子最佳miRNA针头的识别和结合.
- 已证明的集群援助通过DGCR8转录的Drosha切割集成到一个反循环中.
结论:
- 集群miRNA辅助涉及复杂的监管交易,以实现集群miRNA的高效生物发生.
- ERH和SAFB因子在促进微处理器复杂功能的过程中发挥着不同的,有序的作用.
- 一个涉及Drosha分离的DGCR8的反循环调节了微处理器综合体,将集群辅助集成到更广泛的监管网络中.
相关概念视频
01:22MicroRNAs
3.8K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.8K
01:22MicroRNAs
23.9K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.9K
01:57Protein Complexes with Interchangeable Parts
2.9K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.9K
02:30Small interfering RNAs (siRNA)
4.3K
4.3K
02:30siRNA - Small Interfering RNAs
18.4K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
18.4K
02:37Restarting Stalled Replication Forks
6.3K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
6.3K