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Updated: Jul 10, 2026

Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue
08:57

Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue

Published on: July 6, 2011

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研究大鼠大脑正常组织和瘤闪光效应,使用一种新型的非常高能电子束

Tyler V Kay, Anna L Price, Markus Sprenger

    bioRxiv : the preprint server for biology
    |September 18, 2025
    PubMed
    概括

    超高剂量速率 (FLASH) 辐射对癌症治疗有希望. 研究人员开发了一个新的平台来研究FLASH对大脑组织的影响,揭示了正常大脑细胞中潜在的免疫反应.

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    A Micro-CT-based Method for Characterizing Lesions and Locating Electrodes in Small Animal Brains05:12

    A Micro-CT-based Method for Characterizing Lesions and Locating Electrodes in Small Animal Brains

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    相关概念视频

    Overview of Electron Microscopy01:25

    Overview of Electron Microscopy

    The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
    Scanning Electron Microscopy01:07

    Scanning Electron Microscopy

    A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
    Fundamental Principles
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    Transmission Electron Microscopy01:15

    Transmission Electron Microscopy

    In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400 keV in...