Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

599
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
599

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Structure-Guided Optimization of Novel Inhibitors of <i>Plasmodium</i> Lysyl-tRNA Synthetase with Multistage Activity against Malaria Parasites.

Journal of medicinal chemistry·2026
Same author

Vancomycin-resistant Enterococcus often spreads from hospitalized patients into the local environment and less often spreads from the environment into patients.

The Journal of infectious diseases·2026
Same author

End-to-end evaluation of pipelines for metagenome-assembled genomes reveals hidden performance gaps.

bioRxiv : the preprint server for biology·2026
Same author

Blood Pressure Genetic Risk and Incident Hypertension at 2 to 7 Years Post Partum.

JAMA cardiology·2026
Same author

Collateral hypersensitivity between ZY19489 and piperaquine neutralizes PfCRT-mediated drug efflux and Plasmodium falciparum resistance.

Nature communications·2026
Same author

Prevalence and activity of class II microcins in <i>Serratia marcescens</i> strains by isolation source.

Applied and environmental microbiology·2026
Same journal

Layered social competition coordinates reproductive hierarchy formation in ants.

bioRxiv : the preprint server for biology·2026
Same journal

Combination epigenetic-targeted therapy increases the immunogenicity of poorly immunogenic sarcomas.

bioRxiv : the preprint server for biology·2026
Same journal

Loss of LanC-like proteins delays post-injury regeneration of aging skeletal muscles.

bioRxiv : the preprint server for biology·2026
Same journal

Integrative Transfer Network: Deep Transfer Learning Across Populations and Prediction Targets.

bioRxiv : the preprint server for biology·2026
Same journal

Confidence-supported label-free metabolic imaging with FPhaS phase autofluorescence microscopy.

bioRxiv : the preprint server for biology·2026
Same journal

Sequence-encoded autoinhibition couples mRNA decapping activity to phase separation.

bioRxiv : the preprint server for biology·2026
查看所有相关文章

相关实验视频

Updated: Jan 17, 2026

Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing
07:21

Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing

Published on: August 25, 2018

13.4K

在使用宿主遗传资料的微生物组研究中识别样本处理错误.

Julia Urban1, Aya Brown Kav1, William F Kindschuh1

  • 1Program for Mathematical Genomics, Department of Systems Biology, Columbia University Irving Medical Center, New York, NY.

bioRxiv : the preprint server for biology
|September 18, 2025
PubMed
概括
此摘要是机器生成的。

微生物组研究的错误是常见的. 使用通过元基因组测序进行宿主DNA分析的新方法可以检测错误标记的样本,提高数据的准确性和可靠性.

更多相关视频

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
11:22

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

31.1K
A Method for Targeted 16S Sequencing of Human Milk Samples
09:09

A Method for Targeted 16S Sequencing of Human Milk Samples

Published on: March 23, 2018

10.3K

相关实验视频

Last Updated: Jan 17, 2026

Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing
07:21

Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing

Published on: August 25, 2018

13.4K
Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
11:22

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

31.1K
A Method for Targeted 16S Sequencing of Human Milk Samples
09:09

A Method for Targeted 16S Sequencing of Human Milk Samples

Published on: March 23, 2018

10.3K

科学领域:

  • 微生物学 微生物学
  • 遗传学 是一个遗传学.
  • 生物信息学是一种生物信息学.

背景情况:

  • 在大规模的微生物组研究中,样本处理错误很常见,很难检测到.
  • 这些错误可能会损害研究结果的完整性和可靠性.

研究的目的:

  • 介绍两种新的,互补的方法,用于识别微生物组研究中的样本处理错误.
  • 通过强大的错误检测,提高微生物组数据的准确性和可靠性.

主要方法:

  • 利用从元基因组测序中推断出的宿主DNA单核酸多态 (SNP).
  • 将元基因组学推断的SNP与外部基因型化数据 (例如微阵列) 进行比较,以匹配样本与捐赠者.
  • 在样本之间比较由元基因组学推断的SNP,以识别来自同一供体的样本.
  • 将这些方法与实验元数据集成,以增加对错误识别的信心.

主要成果:

  • 在一个纵向的阴道微生物组数据集中成功识别了错误标记的样本.
  • 通过子采样,即使测序覆盖率低,也证明了方法的稳定性.
  • 突出了微生物组研究中处理错误的显著频率.

结论:

  • 本文介绍的基于宿主DNA的方法对于检测微生物组研究中的样本处理错误是有效的.
  • 将SNP比较与元数据分析相结合,为质量控制提供了一个强大的方法.
  • 建议在微生物组研究中实施这些错误检测策略,以确保数据完整性.