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双模式小麦芽聚合素标记 - - 一种用于高分辨率LA-ICP-TOFMS生物成像的多功能细胞分割策略

Claude Molitor^1,2,3, Martin Schaier¹, David Loibnegger^1,2,3

¹Institute of Analytical Chemistry, Faculty of Chemistry, University of Vienna, 1090 Vienna, Austria.

Analytical chemistry

|September 18, 2025

概括

一种新的小麦芽聚合素 (WGA) 标签方法改善了用于激光切除的细胞膜识别,用于感应合等离子体飞行时间质谱学 (LA-ICP-TOFMS) 单细胞分析,提高了准确性和简化工作流程.

科学领域:

生物医学工程生物医学工程
分析化学分析化学
细胞生物学细胞生物学

背景情况:

单细胞分析需要精确细分细胞结构.
目前用于LA-ICP-TOFMS的膜标签协议通常需要组织特定的优化.
精确的细胞细分对于高分辨率的元素映射和表型化至关重要.

研究的目的:

为LA-ICP-TOFMS开发一种广泛适用的和强大的细胞膜标签协议.
为了提高高分辨率元素成像中单细胞细分的准确性和效率.
为现有的膜标签方法提供可扩展和具有成本效益的替代方案.

主要方法:

开发了一种双重标记策略,使用光标记的小麦胚芽聚合素 (WGA) 和金属标记的抗WGA抗体.
应用了基于WGA的协议来对细胞膜和细胞核进行标记.
集成光显微镜和LA-ICP-TOFMS用于交叉验证和高分辨率元素映射.

主要成果:

通过WGA实现了强大,高对比的膜标签,兼容光显微镜和LA-ICP-TOFMS.
证明了在高分辨率下增强的细胞细分精度,促进下游单细胞数据分析.
该协议被证明是广泛适用的,降低了染色成本和简化工作流程.

结论:

基于WGA的双重标签策略为LA-ICP-TOFMS中细胞膜标签提供了多功能和高效的解决方案.
这种方法显著提高了单细胞细分的精度和元素分布分析的质量.
该协议为高分辨率单细胞分析提供了可扩展和成本效益的进步.

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