在中国东部,通过下一代测序在下水道中检测和人类艾契病毒的植物遗传特征,2019-2022年

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概括

爱知病毒 (AiV) 在2019-2022年期间经常在中国的污水中检测到,显示季节性峰值和遗传多样性. 下一代测序 (NGS) 为废水流行病学提供了增强的检测.

科学领域:

环境微生物学环境微生物学
病毒学病毒学
公共卫生监督是对公共卫生的监督.

背景情况:

艾契病毒 (AiV) 引起胃肠炎,污水监测是跟踪流行率的关键.
关于中国的AiV流行病学和遗传多样性的数据有限.
了解废水中的AiV对于公共卫生至关重要.

研究的目的:

为了调查艾滋病毒在济南的流行,季节性和遗传多样性,中国的污水.
评估安普利康下一代测序 (NGS) 对废水中AiV检测的实用性.

主要方法:

在中国济南 (2019-2022) 收集的每月污水样本.
对AiV基因组进行定量分析.
VP1区域放大和下一代测序 (NGS).
对VP1序列的遗传学分析.

主要成果:

高AiV检测率 (95.83%) 的度从1.4×103到2.7×105基因组拷贝/L.
在夏季和秋季观察到的季节性峰值.
遗传学分析显示了A型和B型基因组中的聚类,其中A型基因组占主导地位 (86.06%).
检测到具有显著遗传多样性的多个AiV血统的共同循环.

结论:

在济南的污水中,AiV一直存在,表现出季节性模式和遗传多样性.
阿姆普利康NGS是一种敏感和全面的工具,用于废水中AiV分子流行病学.
调查结果强调了污水监测对监测AiV传播动态的价值.

关键词:

艾病毒艾病毒环境监督环境监督环境监督环境监督环境监督下一代测序的下一代测序.人类的基因组学.污水污水处理污水污水处理

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