相关实验视频

Updated: Jan 17, 2026

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
06:58

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

Published on: October 7, 2021

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一个简单,快速和经济高效的协议,用于超敏感的核糖体分析.

Jiří Koubek1, Katharina Jetzinger1, Shiran Dror1

  • 1Center for Molecular Biology of Heidelberg University (ZMBH) and German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance, Heidelberg, 69120, Germany.

Nucleic acids research
|September 18, 2025
PubMed
概括

我们开发了一种具有成本效益的核糖体分析方法,使用珠合反应. 这种超敏感的协议提高了信使RNA (mRNA) 翻译研究的产量和吞吐量,即使RNA输入最小.

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Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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