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在格类鱼中,Aqp10对应物具有广泛的分离选择性的损失和收益

Ayumi Nagashima^1,2, Shinichiro Hidaka^1,2, Chihiro Ota^1,2

¹School of Life Science and Technology, Institute of Science Tokyo, Yokohama, Kanagawa 226-8501, Japan.

Genome biology and evolution

|September 18, 2025

概括

鱼中的Aquaporin 10 (Aqp10) 基因进化揭示了获得广泛溶解物选择性的类似物. 欧洲鱼Aqp10.2b对象获得了与祖先形式相似的尿素和酸的透性.

科学领域:

分子生物学分子生物学
进化生物学进化生物学
生物化学生物化学

背景情况:

水素10 (Aqp10) 属于水素甘油家族,可以运输水和小无电荷溶液.
目前尚不完全了解水糖素的溶解物选择性机制.
基因重复和丢失事件已经塑造了鱼类中的Aqp10进化.

研究的目的:

研究欧洲鱼中Aqp10.2b类型的功能差异.
为了了解Aqp10的进化历史,在Anguilliformes中具有广泛的溶液选择性.

主要方法:

在欧洲鱼中对Aqp10对应物进行基因分析.
在Xenopus卵细胞中表达Aqp10.2b对应物.
通过卵细胞测量溶解物 (尿素,玻利酸) 的透性.

主要成果:

欧洲鱼拥有三种Aqp10.2b对应物 (Aqp10.2b1-b3),这是由于最近的并联重复.
与Aqp10.2b1.1.3相比,Aqp10.2b2和Aqp10.2b3的芳香/氨酸选择性过器呈现出改变的情况.
Aqp10.2b2和Aqp10.2b3对尿素和酸的透性增加,恢复了广泛的溶液选择性.

结论:

欧洲鱼Aqp10.2b的类似物已经发展出明显的溶液选择性.
欧洲鱼的并列重复导致了Aqp10.2b2和b3.3中广泛的溶解物选择性.
这项研究阐明了鱼类鱼中Aqp10对应物和溶解物运输的进化动态.

关键词:

欧洲鱼欧洲鱼水素10 10 水素的使用方法新子功能化的新子功能化.溶液的选择性溶液的选择性协同复制的双重复制.

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