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三维 (3D) 胰腺β细胞模型揭示了绿色合成银纳米粒子的胰岛素分泌增强潜力

Cigdem Aydin Acar¹, Suray Pehlivanoglu²

¹Department of Health and Biomedical Sciences, Burdur Mehmet Akif Ersoy University, Burdur, Turkey; Department of Nursing, Burdur Mehmet Akif Ersoy University, Bucak School of Health, Burdur, Turkey.

Molecular and cellular endocrinology

|September 18, 2025

概括

从桃茎中合成的银纳米粒子 (Cs-AgNPs) 显示出抗氧化特性,并增强胰腺细胞中的胰岛素分泌. 这些发现表明Cs-AgNP在糖尿病管理中的潜在应用.

科学领域:

纳米技术纳米技术
生物医学科学生物医学科学
糖尿病研究糖尿病研究

背景情况:

银纳米粒子 (AgNPs) 正在探索各种生物医学应用.
自然提取物为纳米粒子合成提供了一个可持续的途径.
胰腺β细胞对于胰岛素生产和糖尿病管理至关重要.

研究的目的:

使用桃茎提取物 (Cs-AgNPs) 合成和表征银纳米颗粒.
为了评估Cs-AgNPs的抗氧化活性.
研究Cs-AgNPs在胰腺β细胞模型中增强胰岛素分泌的潜力.

主要方法:

使用桃茎提取物和银酸盐合成Cs-AgNP.
通过UV-Vis,EDS,TEM和XRD进行表征.
对INS-1和RINm5F细胞系的细胞毒性评估.
使用ABTS测定对抗氧化剂活性的评估.
用3D胰腺β细胞球体测量胰岛素分泌的功能研究.

主要成果:

在大小从10.93到31.18纳米之间成功合成了Cs-AgNP.
在INS-1和RINm5F细胞的特定度下观察到细胞毒性.
Cs-AgNPs表现出显著的抗氧化活性 (IC50 = 78.81 μg/mL).
在3D胰腺β细胞模型中观察到胰岛素分泌量的显著增加 (RINm5F的1.6倍,INS-1的1.2倍).

结论:

桃茎衍生出的银纳米颗粒具有抗氧化特性.
Cs-AgNPs显示了增强胰岛素分泌的潜力.
这些发现凸显了Cs-AgNP在糖尿病相关治疗应用中的前景.

关键词:

3D文化是一个3D文化.具有抗氧化活性的抗氧化剂.桃茎提取物桃茎提取物糖尿病糖尿病糖尿病绿色合成是一种绿色合成.银纳米颗粒 (AgNP) 是一种

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