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通过成像流细胞计量量量化和可视化疟疾感染的红细胞细胞

Takashi Imai¹, Shinya Miyazaki², Yukiko Miyazaki²

¹Department of Parasitology, National Institute of Infectious Diseases (NIID), Japan Institute for Health Security (JIHS), Tokyo, Japan; Department of Infectious Diseases and Host Defense, Gunma University, Gunma, Japan; Department of Microbiology, Saitama Medical University, Saitama, Japan; Leprosy Research Center, NIID, JIHS, Tokyo, Japan; Antimicrobial Resistance Research Center, NIID, JIHS, Tokyo, Japan.

|September 18, 2025

概括

影像流细胞计 (ImageStream) 能够有效地检测小鼠脏和骨髓中的罕见疟疾感染的红细胞细胞 (pEB). 这种强大的技术有助于研究血液组织内疟疾寄生虫相互作用.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
寄生虫学的寄生虫学

背景情况:

疟疾寄生虫传统上感染红细胞.
最近的证据显示,核化红细胞的入侵.
在体内可视化和量化这些寄生性红细胞 (pEBs) 是具有挑战性的.

研究的目的:

采用成像流细胞计 (ImageStream) 来识别和表征pEBs.
为了比较ImageStream与常规流细胞计 (FACS) 用于pEB量化.
为了研究血液构成组织内的宿主-寄生虫相互作用.

主要方法:

用Plasmodium yoelii 17XNL感染的小鼠使用ImageStream和FACS进行了分析.
细胞被染色为红状腺标记物 (TER119),核细胞标记物 (MHC I类,霍克斯特) 和GFP.
分析的重点是脏,骨髓和外周血液.

主要成果:

图像流成功检测并可视化了GFP+TER119+MHC类 Ihi Hoechst+ pEBs.
在脏和骨髓中发现了显著的pEB群体,但不在外周血液中.
图像流量化与FACS相关性很好,验证了成像方法.

结论:

ImageStream是一个强大的工具,用于基于图像的高通量检测pEBs.
这种方法有助于研究血液构成组织中的宿主-寄生虫相互作用.
这种方法可能会揭示对寄生虫持久性和免疫逃避机制的见解.

关键词:

红红质母细胞.图像流的图像流.疟疾:疟疾是一种疾病.青杆菌的叶力杆菌的叶力

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相关概念视频

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...