富含甘油三的条件重塑蛋白质冠状和改变纳米生物相互作用
Arbab Ali1, Mengjie Wang1,2, Haoxiang Guo1,3
1Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China.
ACS applied materials & interfaces
|September 18, 2025
概括
升高的甘油三醇会改变纳米颗粒蛋白冠状形成,增加免疫反应并改变纳米颗粒在体内分布. 这突显了血液代谢物对纳米药物相互作用的影响.
科学领域:
- 纳米医学是一种纳米医学.
- 生物材料科学 生物材料科学
- 免疫学 免疫学 免疫学
背景情况:
- 纳米粒子 (NP) 与生物系统的相互作用是由它们的蛋白质冠状 (PC) 控制的.
- 血液代谢物,如甘油三,对PC形成和随后的纳米生物相互作用的影响仍然在很大程度上未被探索.
- 了解这些相互作用对于开发有效的纳米医学至关重要.
研究的目的:
- 为了研究高甘油 (TG) 水平或高甘油血 (HTG) 对PC成分的影响.
- 评估TG调制PC如何影响NP细胞相互作用和免疫反应.
- 为了确定HTG对NP药理动力学和生物分布的影响.
主要方法:
- 在正常和HTG血清中在NP上形成的PC的蛋白质组分析.
- 使用巨细胞进行体外研究,以评估不同PC的NP的免疫反应 (细胞因子释放).
- 在正常和HTG小鼠的体内生物分布研究.
主要成果:
- 高TG水平促进特定蛋白质的吸附,形成富含TG的PC.
- 与正常PC相比,富含TG的PC刺激了巨细胞中更强的促炎细胞因子释放.
- 在HTG小鼠的肝脏和脏中,NP的积累增加.
- 在HTG血清中形成的PC显示了巨细胞和肝细胞的更高吸收率.
结论:
- 血中代谢物,如甘油三,显著调节PC的形成和NP的生物身份.
- 过高甘油三血症会改变NP诱导的免疫反应和生物分布,影响纳米药物的有效性和安全性.
- 这项研究为设计纳米药物提供了关键的见解,以解释与疾病相关的代谢变化.
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