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灵活的装配,从高速原子力显微镜成像中推断出生物分子中大振幅形态动态的原子精度模型

Romain Amyot¹, Osamu Miyashita², Xuan Wu³

¹WPI Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kanazawa 920-1192, Japan.

|September 18, 2025

概括

高速原子力显微镜 (HS-AFM) 现在为生物分子动力学提供了原子的洞察力. 新的NMFF-AFM方法集成到BioAFMviewer中,从有限分辨率的AFM数据中重建分子电影.

科学领域:

生物物理学的生物物理.
计算生物学计算生物学
显微镜的使用方法

背景情况:

高速原子力显微镜 (HS-AFM) 提供了生物分子过程的动态单分子可视化.
一个关键的限制是从分辨率有限的地形数据中推断出原子细节.
开发计算方法来弥合这个分辨率差距对于理解分子机制至关重要.

研究的目的:

将NMFF-AFM灵活安装方法集成到BioAFMviewer软件平台中.
为了证明这种综合方法的应用,从HS-AFM数据推断出原子模型.
建立一个精简的工作流程来分析HS-AFM成像数据和可视化分子动态.

主要方法:

将NMFF-AFM灵活安装算法的软件集成到BioAFMviewer中.
从原始HS-AFM数据到分析和可视化的直接工作流程的开发.
该方法应用于来自蛋白质域,复合体和细丝的实验HS-AFM数据.

主要成果:

成功将NMFF-AFM集成到BioAFMviewer中,使HS-AFM数据的有效分析成为可能.
证明该方法在各种生物分子系统中复制大幅度的形状运动方面的多功能性.
来自HS-AFM成像序列的蛋白质动态的原子分子电影的重建.

结论:

集成的NMFF-AFM灵活装配方法为从HS-AFM数据中理解生物分子动态提供了强大的工具.
这种用户友好的软件为解释分辨率有限的AFM测量提供了广泛的应用.
产生原子分子膜的能力推动了对生物系统结构变化的研究.

关键词:

3D原子重建3D原子重建灵活的装配AFM灵活的装配飞行机控制软件 (AFM) 是一个软件.高速原子力显微镜蛋白质动力学蛋白质动力学

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