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Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
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The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
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干细胞调节器在植物根部发育过程中驱动G1持续度梯度.

Clara Echevarría1, Bénédicte Desvoyes2, Marco Marconi3

  • 1Centro de Biología Molecular Severo Ochoa, CSIC-UAM, Madrid, Spain.

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概括
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植物根的发育涉及G1持续度梯度,由像PLETHORA这样的干细胞基因和像RETINOBLASTOMA-RELATED 1这样的细胞周期基因调节,影响器官发生.

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科学领域:

  • 植物发育生物学植物发育生物学
  • 细胞周期调节细胞周期调节
  • 根介质系统的动态 根介质系统的动态

背景情况:

  • 机体生成需要协调的细胞增殖和发育计划.
  • 干细胞调节器控制植物体中的增殖,但它们与细胞循环机制的联系尚不清楚.

研究的目的:

  • 为了研究将干细胞调节器与细胞循环机械联系在一起的机制,在阿拉比多普西斯的根 Meristem.
  • 为了确定在根部发育过程中如何控制细胞增殖.

主要方法:

  • 在Arabidopsis根系中分析G1持续度梯度.
  • 对PLETHORA (PLT) 和RETINOBLASTOMA-RELATED1基因进行了基因操纵.
  • 监管网络的计算机建模.监管网络的计算机建模.

主要成果:

  • 在根髓系统 (~2h至>20h) 中确定了近端-远端G1持续度梯度.
  • 在PLT和RETINOBLASTOMA-RELATED1基因的突变取消了这种梯度.
  • PLT基因起到驱动作用,通过KRP5和RETINOBLASTOMA-RELATED1抑制G1的进展.

结论:

  • 在根部发育过程中建立了一个新的近端-远端G1持续梯度.
  • 这种梯度是由干细胞维护和细胞周期调节者的相互作用形成的.
  • G1梯度与对基因组损伤的耐受性增加相关.