补充NADH可以改善受控卵巢高刺激周期中的人类卵细胞成熟和产生的胚胎的发育能力:一项涉及CDK2/GAS6信号通路的试点研究
Qiqi Zhang1,2,3, Han Yang1,2,4, Dandan Yang1,2,3
1Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.
Frontiers in endocrinology
|September 19, 2025
概括
尼古丁胺胺氨基二核酸 (NADH) 补充剂通过增强线粒体功能和调高CDK2和GAS6等关键基因来显著改善人类卵细胞成熟和胚胎发育.
科学领域:
- 生殖生物学 生殖生物学
- 线粒体医学 线粒体医学
- 发展生物学 发展生物学
背景情况:
- 未成熟卵细胞中的线粒体功能障碍是成功体外成熟 (IVM) 的一个主要障碍,特别是在卵巢储备减少的情况下.
- 虽然NAD+前体已得到充分研究,但NADH在人类卵细胞成熟中的直接作用尚不清楚.
研究的目的:
- 研究NADH对人类卵细胞成熟和随后胚胎发育的直接影响.
- 探索NADH对卵细胞质量和功能影响的潜在机制.
主要方法:
- 丢弃的人类卵细胞在IVM介质中培养,具有不同的NADH度,以确定最佳剂量 (10-6M).
- 机制研究包括线粒体功能测试,单细胞RNA测序 (scRNA-seq) 和基因抑制/补充实验.
主要成果:
- NADH显著增加了卵细胞成熟率 (26.31%) 和胚胎细胞形成率 (23.4%).
- 线粒体功能得到改善,由增加的ATP,膜潜力和谷氨,以及减少的活性氧物种 (ROS) 证明.
- NADH提高了CDK2和GAS6的调节;CDK2抑制损害了成熟,而GAS6补充剂提高了胚胎细胞的发育率.
结论:
- NADH作为线粒体生物能增强剂,改善人类卵细胞成熟和胚胎发育.
- NADH的积极作用与CDK2和GAS6的上调相关,突出了它们在卵细胞发育能力中的作用.
相关概念视频
02:07Oogenesis
69.1K
In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...
69.1K
01:22Oogenesis
3.7K
Oogenesis, the process of developing egg cells (female gametes), occurs within the ovaries and is fundamental to female fertility. This sequence begins during fetal development when diploid oogonia in the developing ovaries undergo mitotic divisions to produce primary oocytes. By birth, these primary oocytes enter prophase I of meiosis but become arrested in this stage, remaining suspended until puberty.
Each primary oocyte is surrounded by a layer of pre-granulosa cells, forming what is...
Each primary oocyte is surrounded by a layer of pre-granulosa cells, forming what is...
3.7K
02:02Meiosis II
49.1K
Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
49.1K