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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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相关实验视频

Updated: Jan 17, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
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在活体中分析RNAG-四重复合体.

Bibo Yang1, Yiliang Ding1, Yueying Zhang1

  • 1Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park, Norwich, United Kingdom.

Current protocols
|September 19, 2025
PubMed
概括
此摘要是机器生成的。

通过化学探测,SHALiPE-Seq在活细胞中量化了RNA G-四重复合体 (RG4s). 这种方法将体内RNA折叠与体内基准进行比较,揭示了RG4形成的动态.

关键词:
这是一个G-四重复式.对RNA结构进行分析.沙利佩-塞克 (SHALiPE-Seq) 是一个

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A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
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科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 生物化学 生物化学

背景情况:

  • RNA G-四复合体 (RG4s) 是复杂的三级结构,在富含关氨酸的RNA区域中形成.
  • 在体内检测RG4s一直是具有挑战性的,限制了对它们的生物学作用的理解.

研究的目的:

  • 介绍SHALiPE-Seq,一种用于评估活细胞RG4折叠状态的全转录组方法.
  • 为了能够对RG4形成和细胞环境中的动态进行定量分析.

主要方法:

  • SHALiPE-Seq将化学探测与高通量测序相结合.
  • 它使用2-甲基尼古丁酸伊米达化物 (NAI) 来选择性地修改折叠的RG4s.
  • 在折叠 (K+) 和展开 (Li+) 条件下的体外NAI修改模式作为基准.

主要成果:

  • SHALiPE-Seq允许在体内识别和评估RG4形成.
  • 该方法提供了整个转录组的RG4折叠状态的定量概况.
  • 在Arabidopsis thaliana和大米中成功应用.

结论:

  • SHALiPE-Seq是一个强大的工具,用于研究RG4s的体内动态.
  • 这种方法广泛适用于各种生物系统.
  • 它有助于研究RG4s.s.的潜在生物功能.