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转变的牛热细胞干细胞系,表征,基因编辑和分泌物

Viju Vijayan Pillai^1,2, Prasanthi P Koganti¹, Shailesh Gurung¹

¹Department of Animal Science, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY 14853, USA.

Biology of reproduction

|September 19, 2025

概括

研究人员开发了转换的牛热细胞干细胞 (bTSC) 线,用于研究早期怀孕. 这些细胞系保持了关键特征,并允许进行基因操纵,有助于研究牛胚胎-子宫相互作用.

科学领域:

生殖生物学生殖生物学
干细胞生物学干细胞生物学
哺乳动物发展哺乳动物发展

背景情况:

热原体干细胞 (TSC) 对于理解胎盘发育和怀孕的建立至关重要.
牛皮对于孕期的延长和孕妇对怀孕的认知至关重要.

研究的目的:

为体外研究生成和表征转化牛TSC (bTSC) 线.
建立一个可再生的模型来研究牛的热囊细胞生物学和胚胎-子宫信号.

主要方法:

通过猿真空化病毒40大T抗原的lentiviral转导生成bTSC线.
通过抑制Rho相关蛋白激酶 (ROCK) 维持细胞系.
评估差异化,基因引入 (晶状病毒载体) 和基因编辑 (CRISPR/Cas9);蛋白质组分析.

主要成果:

转换的bTSC线条呈现出快速扩散,并保留了关键的TSC特征.
细胞在TGF-β诱导后经历了与热囊细胞成熟相一致的形态和分子变化.
证明了有效的基因引入和删除,以及识别可能参与胚胎-子宫相互作用的分泌蛋白质.

结论:

转换的bTSC线提供了一个有价值的,可再生的体外模型,用于牛的细胞生物学.
这些细胞系促进了功能性基因研究和对 trofhoblast-endometrial 信号的调查.
该模型系统可以进一步探索牛早期怀孕中的分子机制.

关键词:

胎盘胎盘胎盘胎盘一个胚胎胚胎.基因编辑基因编辑一些秘密一些秘密.干细胞是干细胞的组成部分.

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