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与感染艾滋病毒的人群中病毒抑制的炎症和凝血相关的补充成分的调节失调
Natalie T Subia1, Thomas K Awamura1, Logan S Dean1,2
1Department of Tropical Medicine, Medical Microbiology, and Pharmacology, John A. Burns School Medicine, University of Hawai'i at Mānoa, Honolulu, HI 96813, United States.
Journal of immunology (Baltimore, Md. : 1950)
|September 19, 2025
概括
艾滋病毒感染者 (PLWH) 呈现改变的补体系统 (C2,C5a) 和激活的血小板,促进中性粒细胞外细胞陷 (NETs). 这表明补体失调有助于PLWH的炎症和并发症.
科学领域:
- 免疫学 免疫学 免疫学
- 血液学 血液学 血液学
- 传染性疾病 传染性疾病
背景情况:
- 补体系统,血小板和中性粒细胞都与炎症和血栓形成有关.
- 它们在携带人类免疫缺陷病毒 (PLWH) 的人群中的特定作用仍未得到充分研究.
研究的目的:
- 与HIV阴性个体相比,研究病毒抑制的PLWH中的补充成分,凝血标记物和中性粒细胞激活.
- 确定PLWH血在促进中性粒细胞外细胞陷 (NETs) 和补充因子和血小板的影响中的作用.
主要方法:
- 补充剂 (C2,C3a,C5a,C9) 的量化血水平,凝血标记物和中性粒细胞激活标记物 (MPO,MMP-9).
- 通过流式细胞计量分析了血小板和激活血小板计数 (CD62P+细胞).
- 进行了ex vivoNET测试,以评估NET的形成以及C5aR抗体和血小板去除的影响.
主要成果:
- PLWH的C2和C5a水平显著变化,与蛋白C和MPO相关.
- 艾滋病毒状态预测了C2和C5a水平,在PLWH中血小板上增加了CD62P表达.
- 在健康的中性粒细胞中,PLWH血促进了NET的形成,这种作用通过抑制C5aR和去除血小板而减少.
结论:
- 活化血小板和PLWH中C5a水平升高有助于NET形成.
- 结果表明PLWH的补体调节失调,炎症和凝血变化.
- 这些机制可能会导致PLWH中与年龄相关的非艾滋病并发症.
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