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环境敏感的光探针用于选择性蛋白质的可视化:最近的进展和生物医学应用

Jinhui Hu¹, Lulu Jiang¹, Lanqing Li¹

¹Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen, 529020, PR China.

European journal of medicinal chemistry

|September 19, 2025

概括

对环境敏感的光探测器提供先进的蛋白质可视化,具有高的信号噪声比. 最近的发展侧重于这些化学工具的设计策略,激活机制和生物应用.

科学领域:

化学生物学化学生物学
分子成像学分子成像学

背景情况:

环境敏感的光探头是模块化化学工具,用于可视化特定蛋白质.
它们使用响应性光体,根据微环境极性和粘度改变排放,比传统染料具有优势.

研究的目的:

审查最近 (2020-2025) 在设计,激活机制和对环境敏感光探针的生物应用方面的进展.
概述探测器开发的未来方向,包括NIR-II光体,人工智能辅助发现和体内翻译.

主要方法:

总结最近关于光探针设计策略的文献,重点关注对环境敏感的光体.
分析探头激活机制及其不断扩大的生物应用,包括蛋白质-蛋白质相互作用调节剂和代谢酶.
审查使用启动探针来评估蛋白质分解向化母 (PROTAC) 活动和光PROTAC用于向降解跟踪的使用.

主要成果:

模块化探头设计增强了信号噪声比,并使无洗成像.
应用范围已经超越激酶范围,包括蛋白质-蛋白质相互作用调节剂,代谢酶,道和受体.
光探针越来越多地用于监测PROTAC活动,并针对生物系统的退化.

结论:

对环境敏感的光探测器是一个快速发展的领域,对生物研究和诊断有很大的潜力.
未来的方向包括开发近红外II (NIR-II) 探测器,利用人工智能进行发现,以及改善临床应用的体内翻译.

关键词:

环境敏感的光探测器探测器针对蛋白质的向.开启式光体的光体打开成像系统的启动.

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