碳酸气溶的氧化潜力和细胞毒性在大气模拟室中经历老化
Virginia Vernocchi1, Marco Brunoldi2, Silvia Canepari3
1Istituto Nazionale di Fisica Nucleare, Sezione di Genova, via Dodecaneso 33, 16146 Genoa, Italy.
Toxicology and applied pharmacology
|September 19, 2025
概括
暴露于烟灰颗粒,特别是光照下与二烯接触,会增加氧化潜力和细胞毒性. 衰老的颗粒物可能有缓解作用,突出化学成分和环境衰老在空气污染健康风险中的重要性.
科学领域:
- 环境化学环境化学
- 毒理学 毒理学 毒理学
- 大气科学 大气科学
背景情况:
- 颗粒物 (PM) 空气污染对健康构成重大风险.
- 烟灰颗粒是PM的主要组成部分,它们的毒性受大气老化和环境条件的影响.
研究的目的:
- 研究化学成分,大气老化和环境条件如何影响烟尘颗粒的氧化潜力 (OP) 和细胞毒性.
- 评估OP测试在预测PM引起的健康影响中的作用.
主要方法:
- 使用大气模拟室 (ASC ChAMBRe) 模拟现实世界的夏季和冬季场景.
- 暴露在纯烟尘颗粒和二次气溶前体 (托卢,2,5-二甲基,α-皮) 的光/黑暗条件和氧化剂.
- 使用DCFH,DTT和AA测定评估OP;在BEAS-2B细胞中评估细胞毒性 (ROS,细胞毒性,DNA损伤,IL-8释放).
主要成果:
- 暴露在光线下的烟灰颗粒,特别是烯,显示出更高的OP和增加的细胞毒性,包括ROS产生,细胞毒性,DNA损伤和IL-8释放.
- 硫酸的添加减少了OP,并没有增强毒理反应.
- 在高辐射 (夏季/交通) 条件下产生的烟尘比在低辐射 (冬季) 场景中产生的烟尘有更大的毒性.
- 在OP和毒理终点 (ROS,LDH,微核,IL-8) 之间发现了强烈的相关性.
结论:
- 氧化潜力 (OP) 测定是PM诱导的氧化应激和潜在的健康影响的可靠预测指标.
- 烟灰颗粒的化学成分和环境老化显著影响毒性.
- 了解这些因素对于评估城市空气污染风险和制定减缓策略至关重要.
更多相关视频
08:48Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System
Published on: December 21, 2016
9.1K
12:15Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
Published on: May 29, 2019
9.1K
相关概念视频
01:20Radical Autoxidation
3.1K
The oxidation of an organic compound in the presence of air or oxygen is called autoxidation. For example, cumene reacts with oxygen to form hydroperoxide. Autoxidation involves initiation, propagation, and termination steps. Many organic compounds are susceptible to autoxidation—especially ethers in the presence of oxygen, which form hydroperoxides. Even though this reaction is slow, old ether bottles contain small amounts of peroxide, which leads to laboratory explosions during ether...
3.1K
01:29Oxygen Requirements and Growth Patterns
1.2K
Microorganisms exhibit diverse oxygen requirements and growth patterns driven by their metabolic strategies and environmental adaptations. Oxygen, while essential for many organisms, can also be toxic under certain conditions, shaping how microorganisms grow and survive.Oxygen Requirements of MicroorganismsMicroorganisms are classified based on their ability to use or tolerate oxygen:● Obligate aerobes like Mycobacterium tuberculosis need oxygen for energy production, as it serves as the...
1.2K
01:20Chronic Obstructive Pulmonary Disease-II: Pathophysiology
4.2K
Chronic Obstructive Pulmonary Disease (COPD) pathophysiology is intricate and multifaceted, involving a complex interplay of physiological processes. Understanding these mechanisms is crucial for effectively managing and treating COPD. Here is an in-depth look at the critical elements in the pathophysiology of COPD:
Chronic Inflammation
Chronic Inflammation
4.2K