相关实验视频

Updated: Jan 17, 2026

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

机器学习和功能验证将OsRAV11/12确定为干旱耐受性和大米早期开花的负调节者

You Zhang¹, Muhammad Ikram¹, Naveed Khan²

¹Hainan Yazhou-Bay Seed Laboratory, School of Breeding and Multiplication, Hainan University, Sanya, 572025, China; Collaborative Innovation Center of Nanfan and High-Efficiency Tropical Agriculture, School of Tropical Crops and Forestry, Hainan University, Haikou, 570228, China.

International journal of biological macromolecules

|September 19, 2025

概括

机器学习确定了OsRAV11/12作为大米干旱耐受性的负调节剂. 淘汰线显示出增强的弹性,而过度表达线则易受影响,揭示了改善作物的关键遗传机制.

科学领域:

植物生物学植物生物学
遗传学遗传学是一个
生物技术是生物技术.

背景情况:

米 (Oryza sativa L.) 是一个关键的全球粮食来源,受到干旱压力的威胁.
识别干旱耐受性的遗传调节者对于作物改善至关重要.

研究的目的:

利用机器学习 (ML) 识别调节大米干旱应激反应的关键基因.
为了功能验证候选基因,特别是OsRAV11/12,作为干旱耐受性的调节者.

主要方法:

训练随机森林,XGBoost和Feedforward神经网络模型在米的转录基因数据上.
在基因淘汰 (KO) 和过度表达 (OX) 实验中使用CRISPR/Cas9.
进行了生理和转录组分析,以评估干旱反应.

主要成果:

XGBoost模型在预测干旱耐受性基因方面取得了90%的准确性.
OsRAV11/12被确定为负调节剂;KO线显示80-85%的存活率与野生类型的57.5%相比.
KO 线表现出改善的生理特征 (更低的口腔导电率,更高的 ABA) 和应激反应基因表达.

结论:

OsRAV11/12对大米的干旱耐受性进行负面调节.
机器学习是一个强大的工具,可以加速发现控制复杂特征的基因,例如抗旱能力.
研究结果为开发适应气候变化的米品种提供了目标.

关键词:

耐旱耐受性耐旱耐受性耐旱耐受性机器学习机器学习转录因子 OsRAV 的转录因子大米大米大米大米大米在SHAP分析中,我们分析了SHAP.

更多相关视频

A Telemetric, Gravimetric Platform for Real-Time Physiological Phenotyping of Plant–Environment Interactions

A Telemetric, Gravimetric Platform for Real-Time Physiological Phenotyping of Plant–Environment Interactions

Published on: August 5, 2020

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Published on: September 2, 2019

相关概念视频

Responses to Drought and Flooding

Responses to Drought and Flooding

Water plays a significant role in the life cycle of plants. However, insufficient or excess of water can be detrimental and pose a serious threat to plants.

Adaptations that Reduce Water Loss

Adaptations that Reduce Water Loss

Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.

Responses to Salt Stress

Responses to Salt Stress

Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.

Regulation of Transpiration by Stomata

Regulation of Transpiration by Stomata

During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.

Plant Breeding and Biotechnology

Plant Breeding and Biotechnology

Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.