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和雌激醇调节了Actinobacillus seminis中的蛋白酶和血红蛋白酶的表达

Gerardo A Ramírez-Paz-Y-Puente¹, José A Gutiérrez-Pabello², Edgar Zenteno³

¹Carrera de Biología, Facultad de Estudios Superiores Iztacala, UNAM, Av. de los Barrios 1, Los Reyes Iztacala, Tlalnepantla 54090, Estado de México, México.

FEMS microbiology letters

|September 19, 2025

概括

像丸激素和雌激素这样的性激素通过增加蛋白酶表达来增加Actinobacillus seminis的毒性. 这一发现突出了荷尔蒙.

科学领域:

兽医微生物学兽医微生物学
细菌病原体的产生
内分泌学在内分泌学.

背景情况:

细菌 Actinobacillus seminis 在反动物中引起生殖系统疾病.
性激素影响A.seminis的毒性因素,生长和生物膜的形成.
对A.seminis蛋白酶表达的荷尔蒙影响仍未得到研究.

研究的目的:

为了研究和雌醇对A.seminis蛋白酶表达的影响.
描述性质并识别由性激素调节的A.seminis蛋白酶.

主要方法:

用 (1-5 ng/ml) 和雌激醇 (5-25 pg/ml) 对A.seminis的治疗.
细胞图分析以检测蛋白酶活性.
生物化学表征 (pH,温度稳定性) 和基质水解试验.
免疫学交叉反应性测试.

主要成果:

和雌激醇都增强了50kDa的金属蛋白酶和65kDa的血清蛋白酶的分泌.
65kDa的血清蛋白酶在pH6-8时表现出最佳活性,在高达70°C的温度下稳定,以及化血红蛋白和素.
这种血红蛋白酶是由性类固醇诱导的,而不是甲基醇胺.
金属蛋白酶水解了IgG和纤维素原.
这两种蛋白质酶都与抗菌素对相关的细菌蛋白质酶产生交叉反应.

结论:

性激素显著调节关键的A.seminis毒性因子的表达,特别是金属蛋白酶和蛋白酶.
这些激素诱导的蛋白酶可能会导致反动物A.seminis感染的发病.
激素调节毒性因子是控制A. seminis相关疾病的潜在目标.

关键词:

这种细菌是Actinobacillus seminis.卡塞因 (casein) 是一种蛋白质.雌二醇是雌二醇的一种药物.血红蛋白血红蛋白是一个蛋白质酶蛋白质酶是一种蛋白质.测试剂测试剂测试剂测试剂

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