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Updated: Jan 17, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
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融合 (FUSION):一种家族层面的整合方法,用于对小型非编码RNA的强有力的差异分析

Hukam C Rawal1, Qi Chen2,3, Tong Zhou1

  • 1Department of Physiology and Cell Biology, University of Nevada, Reno School of Medicine, Reno, NV 89557, United States.

Bioinformatics (Oxford, England)
|September 19, 2025
PubMed
概括

FUSION是一种新的计算工具,通过将独特的物种整合到父母RNA家族中来分析非正规的小型非编码RNA (sncRNA). 这种方法提高了差异丰度分析的统计能力,即使样本大小小.

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
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RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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