基于血的生物传感器在控制细菌行为中的作用
Florian J Fekete1, Emily E Weinert1
1Department of Biochemistry & Molecular Biology, The Pennsylvania State University, University Park, PA 16802, United States.
Journal of inorganic biochemistry
|September 19, 2025
概括
细菌中的基于血红素的传感器检测到像氧气这样的气体,影响细胞过程. 全球蛋白合传感器 (GCS) 是关键的血红蛋白,参与氧气传感和循环-二-瓜诺辛单酸盐 (c-di-GMP) 信号通路.
科学领域:
- 生物化学 生物化学
- 微生物学 微生物学
- 分子生物学分子生物学
背景情况:
- 血红系辅因子在生命中至关重要,在气体运输,催化和传感中发挥作用.
- 基于血的传感器检测到氧化 (NO),一氧化碳 (CO) 和氧 (O2) 等气体,调节细胞反应.
- 细菌的氧气反应涉及多种类型的血传感器,包括含血的Per-Arnt-Sim (hPAS) 和传感器球蛋白域.
研究的目的:
- 研究血红蛋白在细菌氧气反应中的作用.
- 探索全球合传感器 (GCS) 蛋白质作为一种广泛的基于血红素的O2传感器家族.
- 了解GCS蛋白质作为循环-二-瓜诺辛单酸盐 (c-di-GMP) 信号传递的模型.
主要方法:
- 血红蛋白的生物化学表征.
- 在GCS蛋白质中对联体结合和选择性的分析.
- 研究不同氧气传感系统 (例如hPAS和GCS) 之间的相互作用.
主要成果:
- GCS 蛋白稳定 O2 结合,并介导连接体选择性信号传递.
- 具有迪加尼酸环酶 (DGC) 域的GCS蛋白在O2检测时合成c-di-GMP.
- 具体的例子,如 EcDosC, PccDgcO 和 BpeGReg 证明了 GCS 的功能.
- 大肠杆菌hPAS (DosP) 和GCS (DosC) 之间的相互作用揭示了复杂的氧气调节网络.
结论:
- 全球蛋白合传感器代表着一种重要的细菌血红素基氧传感器家族.
- GCS蛋白质将氧气检测与下游信号通路联系起来,特别是c-di-GMP合成.
- 血传感器的复杂相互作用突出了细菌对环境氧气水平的复杂适应.
相关概念视频
Oxygen Requirements and Growth Patterns
1.2K
Microorganisms exhibit diverse oxygen requirements and growth patterns driven by their metabolic strategies and environmental adaptations. Oxygen, while essential for many organisms, can also be toxic under certain conditions, shaping how microorganisms grow and survive.Oxygen Requirements of MicroorganismsMicroorganisms are classified based on their ability to use or tolerate oxygen:● Obligate aerobes like Mycobacterium tuberculosis need oxygen for energy production, as it serves as the...
1.2K
Global Regulatory Systems
612
Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
612
Bacterial Signaling
40.1K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
40.1K
Physiological Control of Respiration
5.9K
Introduction
Breathing, a seemingly passive process, is regulated by the respiratory center in the brainstem. This center coordinates the involuntary control of respirations, which means it occurs without conscious effort, ensuring a smooth and uninterrupted pattern.
Regulation of Ventilation
The body maintains ventilation by monitoring levels of carbon dioxide (CO2), oxygen (O2), and hydrogen ion concentration (pH) in the arterial blood. Among these factors, the level of CO2 plays a crucial...
Breathing, a seemingly passive process, is regulated by the respiratory center in the brainstem. This center coordinates the involuntary control of respirations, which means it occurs without conscious effort, ensuring a smooth and uninterrupted pattern.
Regulation of Ventilation
The body maintains ventilation by monitoring levels of carbon dioxide (CO2), oxygen (O2), and hydrogen ion concentration (pH) in the arterial blood. Among these factors, the level of CO2 plays a crucial...
5.9K
Gene Regulation in Microbial Communities: Quorum Sensing
533
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
533
Chemical Factors Affecting Respiration Centers
2.1K
Chemical factors such as changing CO2, O2, and H+ levels in arterial blood play a critical role in influencing respiration depth and rates. These variations are detected by chemoreceptors—specialized sensors located in two primary body areas. Central chemoreceptors are found throughout the brain stem, including the ventrolateral medulla, while peripheral chemoreceptors are located in the aortic arch and carotid arteries.
CO2 has a potent influence on respiration and is strictly regulated....
CO2 has a potent influence on respiration and is strictly regulated....
2.1K


