在不同的碳来源和电子受体下,产生曼尼托尔的果实菌株的果实性代谢
Florencia Mohamed1, Luciana G Ruiz Rodríguez1, Luc De Vuyst2
1Centro de Referencia para Lactobacilos (CERELA)-CONICET, Chacabuco 145, 4000 San Miguel de Tucumán, Tucumán, Argentina.
Journal of applied microbiology
|September 19, 2025
概括
果菌菌株有效地从果糖中产生低热量糖曼尼托尔. 通过控制糖度和避免作为电子受体竞争的酸盐,优化曼尼托尔的生产.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 代谢工程是代谢工程.
背景情况:
- 果菌种利用果糖作为能量和电子受体,通过曼尼托尔2-脱酶 (MDH) 生产曼尼托尔.
- 了解Fructobacillus的代谢途径和遗传调节对于优化曼尼托产量至关重要.
研究的目的:
- 研究Fructobacillus sp.的生理和生化特征. CRL 2054 和 F. tropaeoli CRL 2034 . 这两种类型的标志物.
- 通过研究各种碳来源,糖度和电子受体的影响来增强曼尼托尔生产.
主要方法:
- 在不同条件下 (碳来源,糖度,电子受体) 测试微生物生长和曼尼托尔产量.
- 在碳水化合物代谢中涉及的特定的酶活动和基因表达 (果糖酶 (FK),葡萄糖-6-酸盐异构酶 (GPI),MDH).
- 分析了相关基因的基因组背景.
主要成果:
- 两种Fructobacillus菌株都表现出高透耐受性,生长高达40%的碳水化合物.
- 在糖度超过20%时,曼尼托尔的产量下降,并且当pyruvate作为电子受体竞争时,它也下降了.
- MDH基因表达上调,而FK和GPI基因在葡萄糖下调,表明调节果糖代谢以产生曼尼托尔.
结论:
- 果菌菌株具有耐氧性,曼尼托尔可能作为兼容溶液.
- 酸盐通过与果糖作为电子受体竞争来抑制曼尼托尔的产生.
- 在葡萄糖的存在下,果糖通过调节基因表达被有效地降低为曼尼托尔,为增强曼尼托尔产量提供了一种策略.
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