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Updated: Jan 17, 2026

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
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单分子FRET和活细胞中转化生物分子的跟踪

Abhinaya Anandamurugan1, Antonia Eidloth1, Veronika Frank1

  • 1Institute of Physical Chemistry, University of Freiburg, Freiburg, Germany.

Biophysical journal
|September 20, 2025
PubMed
概括

研究人员开发了一种新方法,使用弗斯特共振能量转移 (FRET) 来追踪活细胞内的蛋白质动力学. 该技术允许实时详细分析蛋白质构造变化和细胞局部化.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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