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Pseudomonas sp. 的亚降解酶. PNPG3:一种生物信息学和分子模拟研究,用于阿佐染料排毒

Sk Aftabul Alam¹, Pradipta Saha²

¹Department of Botany, Netaji Mahavidyalaya, Arambagh, West Bengal, India.

Journal of biomolecular structure & dynamics

|September 20, 2025

概括

这项研究确定了来自Pseudomonas sp.的阿佐雷德克塔酶酶. 菌株PNPG3作为降解合成染料的有效剂. 这些酶表现出高稳定性和强大的结合亲和力,为染料污染的生物修复提供了一个有希望的解决方案.

科学领域:

环境科学环境科学
生物技术是生物技术.
生物化学生物化学

背景情况:

合成染料由于其持久性和毒性而带来重大环境风险.
工业染料的使用,特别是在织品中,导致了广泛的生态污染.
使用酶的生物修复提供了一种可持续的方法来减轻染料污染.

研究的目的:

为了研究来自*Pseudomonas* sp.的阿佐降解酶 (Azo) 酶的潜力. 菌株PNPG3用于降解合成色剂.
描述已识别的酶的结构性质和稳定性.
评估这些酶对特定的色素的结合亲和力和降解效率.

主要方法:

对*伪蒙* sp. 的基因组分析. 菌株PNPG3用于识别阿佐基因.
使用计算工具 (ERRAT,PROCHECK,QMEAN4) 对阿佐酶的结构建模和验证.
分子对接模拟以评估与色剂的结合亲和力 (溶剂黑3,刚果红) 和稳定性分析 (RMSD,RMSF,PCA).

主要成果:

鉴定出了两种不同的阿佐基因,根据它们的结构模型,它们表现出高温稳定性.
溶剂黑3与-1具有强烈的结合亲和力 (-10.1 kcal/mol),而刚果红色有效地与-2 (-9.4 kcal/mol) 结合.
该Azo2-Solvent Black 3复合体表现出优越的结构稳定性,表明有效的染料相互作用和降解潜力.

结论:

* 伪蒙 * sp. 的病毒. 菌株PNPG3通过其阿佐酶具有强大的阿佐染料降解能力.
鉴定到的阿佐酶具有热稳定性,并且对色剂具有显著的结合亲缘关系.
这种菌株对开发有效的生物修复策略来应对工业染料污染具有相当大的前景.

关键词:

亚降解酶是一种亚降解酶.伪omonas 是一个类型的.分子对接的分子对接.分子动力学模拟模拟合成染料是一种合成染料.

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