肺损伤中的组织因子驱动的凝血和炎症:来自体外和体外模型的见解
Aleena Varughese1, Akarsha Balnadupete2, Fathimath Muneesa Moideen3
1Cell Biology and Molecular Genetics Division, Yenepoya Research Center, Yenepoya (Deemed to be University), Mangalore, India.
IUBMB life
|September 20, 2025
概括
组织因子 (TF) 在肺损伤和纤维化中驱动血栓炎症. 这项研究揭示了TFTF.
科学领域:
- 肺部医学和病理学
- 血液学和凝血学研究
- 分子生物学和免疫学 分子生物学和免疫学
背景情况:
- 肺损伤和纤维化涉及炎症和凝血之间的复杂相互作用.
- 组织因子 (TF) 启动了外部凝血级联,但其在肺纤维素沉积中的作用尚未完全理解.
- 肺纤维化 (PF) 的进展与失调的凝血途径有关.
研究的目的:
- 研究TF在调解凝血驱动的炎症和肺损伤中的纤维素沉积中的作用.
- 阐明导致肺纤维化的TF介导分子机制.
- 探索潜在的治疗目标,以减轻TF驱动的肺病理.
主要方法:
- 在体外 (A549细胞,Beas2b细胞) 和体内 (C57BL/6小鼠) 模型中使用了白血素 (BLM),TGF-β和TF.
- 综合蛋白质-蛋白质相互作用 (PPI) 网络和基因丰富分析.
- 进行基因表达分析,免疫光和组织学评估 (H&E,Masson Trichrome).
主要成果:
- 肺上皮细胞中凝血因子 (CF-III,CF-VII,CF-X) 的表达增加,A549细胞中的前凝血活性增加.
- 观察到炎症性细胞因子 (IL-6,TNF-α,IL-1β) 的升高以及显著的TF和TNF-α上调.
- 组织学分析证实了小鼠肺组织的炎症和纤维变化,与TF活性相关.
结论:
- 组织因子 (TF) 是血栓炎性肺损伤和肺纤维化的一个关键分子驱动因素.
- TF调解炎症,纤维素沉积,内皮功能障碍和纤维细胞重塑.
- 向TF为治疗肺损伤和纤维化提供了潜在的治疗策略.
相关概念视频
01:20Extrinsic and Intrinsic Pathways of Hemostasis
12.1K
Blood clotting or coagulation involves extrinsic and intrinsic pathways, which ultimately merge into the common pathway, forming a fibrin clot.
The Extrinsic Pathway
The extrinsic pathway of coagulation is typically initiated by tissue damage that exposes blood to tissue factor (TF), a protein released by the damaged tissue cells outside the blood vessels—this interaction with TF triggers biochemical reactions involving specific clotting factors. The key player here is Factor VII, which...
The Extrinsic Pathway
The extrinsic pathway of coagulation is typically initiated by tissue damage that exposes blood to tissue factor (TF), a protein released by the damaged tissue cells outside the blood vessels—this interaction with TF triggers biochemical reactions involving specific clotting factors. The key player here is Factor VII, which...
12.1K
01:09Coagulation
9.7K
The coagulation phase is a critical part of the body's process to prevent blood loss following injury to blood vessels. It involves chemical reactions that form a clot to seal the injured area. The clotting process begins shortly after injury, within 15-20 seconds for severe damage and 1-2 minutes for minor injuries.
During the coagulation phase, clotting factors, or procoagulants, play a vital role in initiating and progressing the coagulation cascade. This cascade is a series of reactions...
During the coagulation phase, clotting factors, or procoagulants, play a vital role in initiating and progressing the coagulation cascade. This cascade is a series of reactions...
9.7K
01:16Clot Retraction and Fibrinolysis
8.5K
After a fibrin clot is formed, the next step is clot retraction, a vital process facilitated by platelet contractile proteins, such as actin and myosin. These proteins pull the fibrin strands closer together and condense the clot. This action reduces the size of the clot, creating a smaller, denser structure that effectively seals off the damaged vessel. Clot retraction consolidates the clot and helps with wound healing by bringing the edges of the damaged blood vessel closer together.
8.5K
01:28Phases of Wound Repair
7.8K
Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
7.8K