在葡萄牙蝙蝠病毒多样性的metagenomic分析
Mahima Hemnani1, Mustafa Karatas2, Andreia V S Cruz1
1School of Medicine and Biomedical Sciences, Porto University, Porto, 4050-313, Portugal.
Veterinary research communications
|September 20, 2025
概括
这项研究在葡萄牙蝙蝠中发现了两种新型冠状病毒 (CoV). 它们的尖蛋白显示出跨物种传播到猪和人类的潜力,突出显示了蝙蝠病毒和新出现的疾病风险.
科学领域:
- 病毒学 病毒学
- 动物传播疾病 动物传播疾病
- 哺乳动物病毒学
背景情况:
- 蝙蝠是动物传播病毒,包括冠状病毒 (CoV) 的重要储存库.
- 了解蝙蝠病毒对新兴传染病监测至关重要.
- 蝙蝠在病毒传播中的生态作用需要持续调查.
研究的目的:
- 在葡萄牙蝙蝠中检测冠状病毒 (CoV).
- 使用元基因组测序来描述蝙蝠样本中的病毒多样性.
- 为了预测蝙蝠CoV尖峰蛋白与宿主受体的结合亲和力.
主要方法:
- 在葡萄牙中部从十只蝙蝠 (Myotis myotis和Miniopterus schreibersii) 采集样本.
- 使用泛CoV嵌套RT-PCR和病毒元基因组测序进行查.
- 在 silico 蛋白质对接,以评估尖端蛋白与蝙蝠,猪和人类氨基酶N (APN) 受体的亲和力.
主要成果:
- 三个样本对Covs呈阳性;两个几乎完整的Alphacoronavirus基因组被测序.
- 大基因组测序揭示了皮科纳病毒,腺病毒和依赖帕尔沃病毒的存在.
- 蛋白质对接表明蝙蝠Cov尖端蛋白与蝙蝠,猪和人类的APN受体有很强的结合.
结论:
- 这项研究报告了葡萄牙蝙蝠的第一个近乎完整的Alphacoronavirus基因组.
- 这些蝙蝠的多样化病毒组包括多个病毒家族.
- 预测的跨物种传播潜力凸显了对蝙蝠传播的CoV与其他物种相互作用的进一步研究的需要.
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