甲沙辛酸乙醇需要甲醇:辅酶M甲基转移酶,以从乙醇中形成乙
Tejas Somvanshi1, Mai Anh Tran1, Jichen Bao1
1Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, 02150, Espoo, Finland.
Antonie van Leeuwenhoek
|September 20, 2025
概括
在Methanosarcina acetivorans中的Corrinoid依赖的甲醇酶可以处理乙基,从乙醇中产生微量乙. 这一发现扩大了对代谢网络和潜在的生物源乙来源的理解.
科学领域:
- 生物化学 生物化学
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 科里诺依赖酶通常处理甲基组转移或基因生成.
- 甲醇特异性甲基转移酶被认为是甲基组独有的.
- 在Methanosarcina中从乙醇中产生微量乙表明了酶乱交.
研究的目的:
- 为了研究一种假设,即依赖于腐化物的甲醇甲基转移酶接受乙烯基组.
- 为了阐明生物化学途径,从乙醇中产生微量乙,在Metanosarcina acetivorans.
- 为了确认MtaCB,MtaA和Mcr酶的基质杂交性.
主要方法:
- 研究了甲醇转化为甲代谢的同源反应,用于乙醇转化.
- 使用MtaB (甲醇甲基转移酶) 来激活乙醇,并将乙烯基组加载到MtaC上.
- 在mtaCB双删除菌株和静止细胞悬浮液中量化乙的生产.
主要成果:
- 甲醇甲基转移酶 (MtaB) 激活乙醇,将乙基转移到MtaC.
- 在MtaA和Mcr中基质杂交对于乙生产也是必要的.
- 乙产量大约比甲产量低三个数量级.
- 在三种MtaCB异酶中证实了乙烯转移能力.
- 乙形成需要先在甲醇上生长才能诱导mtaCB基因表达.
结论:
- 甲醇特异性甲基转移酶表现出对乙基的乱交性.
- 这一发现扩大了已知的代谢网络中的酶反应的范围.
- 建议生物源性乙自然生产的潜在途径.
更多相关视频
04:32Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples
Published on: October 14, 2021
3.1K
06:17Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions
Published on: August 15, 2019
29.6K
相关概念视频
01:33Carboxylic Acids to Methylesters: Alkylation using Diazomethane
2.8K
Carboxylic acids react with diazomethane in an ether solvent via alkylation at the carboxylate oxygen atom to give methyl esters of the corresponding acid with excellent yields.
2.8K
02:35Ethers from Alkenes: Alcohol Addition and Alkoxymercuration-Demercuration
8.9K
Overview
Ethers can also be prepared from alkenes through acid-catalyzed addition of alcohols and alkoxymercuration–demercuration.
Preparation of Ethers by Acid-Catalyzed Addition of Alcohol to Alkenes
The acid-catalyzed addition of alcohol to an alkene involves treating the alkene with an excess of alcohol in the presence of an acid catalyst to form an ether under suitable conditions. The hydrogen will add to the less substituted carbon so that the nucleophile can attack the more substituted...
Ethers can also be prepared from alkenes through acid-catalyzed addition of alcohols and alkoxymercuration–demercuration.
Preparation of Ethers by Acid-Catalyzed Addition of Alcohol to Alkenes
The acid-catalyzed addition of alcohol to an alkene involves treating the alkene with an excess of alcohol in the presence of an acid catalyst to form an ether under suitable conditions. The hydrogen will add to the less substituted carbon so that the nucleophile can attack the more substituted...
8.9K
01:23Microbial Fermentation
1.3K
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
1.3K
01:20Fates of Pyruvate
10.3K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
10.3K
01:15Pyruvate Oxidation
168.1K
After glycolysis, the charged pyruvate molecules enter the mitochondria via active transport and undergo three enzymatic reactions. These reactions ensure that pyruvate can enter the next metabolic pathway so that energy stored in the pyruvate molecules can be harnessed by the cells.
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
168.1K
01:29Overview of Archaea
721
Archaea, named after the Archaean eon, represent a unique domain of life, distinct from bacteria and eukaryotes, with remarkable traits. Their cellular and molecular features, ecological adaptability, and industrial relevance highlight their importance in understanding life processes and leveraging biotechnology.Cellular and Molecular CharacteristicsA defining feature of archaea is their unique membrane composition. Archaeal membranes contain ether-linked isoprenoid lipids, which confer...
721