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活跃卫生垃圾填埋场基线微生物群中的微生物多样性和功能潜力的季节性动态

George Obinna Akuaka¹, Hazzeman Haris², Kamarul Zaman Zarkasi¹

¹School of Biological Sciences, Universiti Sains Malaysia, 11800, Pulau Pinang, Malaysia.

|September 20, 2025

概括

垃圾填埋中的微生物群落因季节性变化而变化,影响了粘土的完整性. 了解这些垃圾填埋场微生物组是改善废物结和地下水保护的关键.

科学领域:

环境微生物学环境微生物学
地质微生物学的生物.
废物管理科学废物管理科学

背景情况:

卫生垃圾填埋场使用工程层来防止污染,但长期的粘土基线层退化会给环境带来风险.
压缩粘土基线 (CCL) 对于垃圾填埋非常重要,但随着时间的推移,它们的结构完整性可能会受到损害.

研究的目的:

调查垃圾填埋场漂水和CCL中的季节性和息地特定的微生物群落.
了解这些微生物的功能作用及其对粘土屏障性能的影响.
评估物理化学参数对微生物社区结构和功能的影响.

主要方法:

使用了16S rRNA基因扩增序列和PICRUSt2功能预测.
液和CCL (0-30厘米深) 的样本是根据季节 (雨季和干旱) 收集的.
与微生物分析一起,测量了物理化学特性和透性.

主要成果:

在液和基线物体物理化学性质中观察到显著的季节性差异.
基线微生物组比液微生物组更稳定,由环境参数驱动的变化.
核心细菌系包括Pseudomonadota,Bacillota和Actinobacteriota;无氧系与矿物转化和潜在的粘土降解有关.

结论:

垃圾填埋场微生物群落表现出生态和功能复杂性,可能会损害屏障性能.
季节性变化对垃圾填埋场的微生物群及其功能有很大影响.
建议对微生物功能基因进行常规监测,并开发弹性粘土材料,以加强长期制.

关键词:

16S rRNA 测序的测序方法环境安全环境安全微生物社区的微生物社区.卫生垃圾填埋场卫生垃圾填埋场季节性变化季节性变化废物管理废物管理

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